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TitleStructural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 7940, Year 2023
Publish dateDec 1, 2023
AuthorsDawei Sun / Yonglian Sun / Eric Janezic / Tricia Zhou / Matthew Johnson / Caleigh Azumaya / Sigrid Noreng / Cecilia Chiu / Akiko Seki / Teresita L Arenzana / John M Nicoludis / Yongchang Shi / Baomei Wang / Hoangdung Ho / Prajakta Joshi / Christine Tam / Jian Payandeh / Laëtitia Comps-Agrar / Jianyong Wang / Sascha Rutz / James T Koerber / Matthieu Masureel /
PubMed AbstractThe C-C motif chemokine receptor 8 (CCR8) is a class A G-protein coupled receptor that has emerged as a promising therapeutic target in cancer. Targeting CCR8 with an antibody has appeared to be an ...The C-C motif chemokine receptor 8 (CCR8) is a class A G-protein coupled receptor that has emerged as a promising therapeutic target in cancer. Targeting CCR8 with an antibody has appeared to be an attractive therapeutic approach, but the molecular basis for chemokine-mediated activation and antibody-mediated inhibition of CCR8 are not fully elucidated. Here, we obtain an antagonist antibody against human CCR8 and determine structures of CCR8 in complex with either the antibody or the endogenous agonist ligand CCL1. Our studies reveal characteristic antibody features allowing recognition of the CCR8 extracellular loops and CCL1-CCR8 interaction modes that are distinct from other chemokine receptor - ligand pairs. Informed by these structural insights, we demonstrate that CCL1 follows a two-step, two-site binding sequence to CCR8 and that antibody-mediated inhibition of CCL1 signaling can occur by preventing the second binding event. Together, our results provide a detailed structural and mechanistic framework of CCR8 activation and inhibition that expands our molecular understanding of chemokine - receptor interactions and offers insight into the development of therapeutic antibodies targeting chemokine GPCRs.
External linksNat Commun / PubMed:38040762 / PubMed Central
MethodsEM (single particle)
Resolution2.76 - 3.1 Å
Structure data

41370

8tlm

EMDB-41370, PDB-8tlm:
Structure of a class A GPCR/Fab complex
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-41827: Structure of a class A GPCR/agonist complex (focused map2)
Method: EM (single particle) / Resolution: 2.76 Å

EMDB-41828: Structure of a class A GPCR/agonist complex (focused map1)
Method: EM (single particle) / Resolution: 2.88 Å

41829

8u1u

EMDB-41829, PDB-8u1u:
Structure of a class A GPCR/agonist complex
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-41850: Structure of a class A GPCR/agonist complex (Consensus map)
Method: EM (single particle) / Resolution: 2.96 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
  • aequorea victoria (jellyfish)
  • human adenovirus c serotype 2
  • mus musculus (house mouse)
KeywordsSTRUCTURAL PROTEIN / GPCR Fab / GPCR / agonist

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