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TitleCryo-EM-based discovery of a pathogenic parvovirus causing epidemic mortality by black wasting disease in farmed beetles.
Journal, issue, pagesCell, Vol. 187, Issue 20, Page 5604-5619.e14, Year 2024
Publish dateOct 3, 2024
AuthorsJudit J Penzes / Martin Holm / Samantha A Yost / Jason T Kaelber /
PubMed AbstractWe use cryoelectron microscopy (cryo-EM) as a sequence- and culture-independent diagnostic tool to identify the etiological agent of an agricultural pandemic. For the past 4 years, American insect- ...We use cryoelectron microscopy (cryo-EM) as a sequence- and culture-independent diagnostic tool to identify the etiological agent of an agricultural pandemic. For the past 4 years, American insect-rearing facilities have experienced a distinctive larval pathology and colony collapse of farmed Zophobas morio (superworm). By means of cryo-EM, we discovered the causative agent: a densovirus that we named Zophobas morio black wasting virus (ZmBWV). We confirmed the etiology of disease by fulfilling Koch's postulates and characterizing strains from across the United States. ZmBWV is a member of the family Parvoviridae with a 5,542 nt genome, and we describe intersubunit interactions explaining its expanded internal volume relative to human parvoviruses. Cryo-EM structures at resolutions up to 2.1 Å revealed single-strand DNA (ssDNA) ordering at the capsid inner surface pinned by base-binding pockets in the capsid inner surface. Also, we demonstrated the prophylactic potential of non-pathogenic strains to provide cross-protection in vivo.
External linksCell / PubMed:39208798 / PubMed Central
MethodsEM (single particle)
Resolution2.0 - 3.4 Å
Structure data

41106

8t9c

EMDB-41106, PDB-8t9c:
Zophobas morio black wasting virus strain UT-morio virion structure
Method: EM (single particle) / Resolution: 2.7 Å

41108

8t9e

EMDB-41108, PDB-8t9e:
Zophobas morio black wasting virus strain UT-morio empty capsid structure
Method: EM (single particle) / Resolution: 2.9 Å

41125

8t9x

EMDB-41125, PDB-8t9x:
Zophobas morio black wasting virus strain NJ2-molitor virion structure
Method: EM (single particle) / Resolution: 2.0 Å

41131

8ta7

EMDB-41131, PDB-8ta7:
Zophobas morio black wasting virus strain OR-molitor empty capsid structure
Method: EM (single particle) / Resolution: 2.7 Å

41301

8tje

EMDB-41301, PDB-8tje:
Zophobas morio black wasting virus strain OR-molitor virion structure
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • zophobas morio (beetle)
  • zophobas morio black wasting virus
  • tenebrio molitor (yellow mealworm)
KeywordsVIRUS/DNA / Capsid / Virion / Parvovirus / Densovirus / Invertebrate / Insect / Pathogen / ssDNA / VIRUS / VIRUS-DNA complex

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