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TitleStructural basis of agonist specificity of α-adrenergic receptor.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 4819, Year 2023
Publish dateAug 10, 2023
AuthorsMinfei Su / Jinan Wang / Guoqing Xiang / Hung Nguyen Do / Joshua Levitz / Yinglong Miao / Xin-Yun Huang /
PubMed Abstractα-adrenergic receptors (α-ARs) play critical roles in the cardiovascular and nervous systems where they regulate blood pressure, cognition, and metabolism. However, the lack of specific agonists ...α-adrenergic receptors (α-ARs) play critical roles in the cardiovascular and nervous systems where they regulate blood pressure, cognition, and metabolism. However, the lack of specific agonists for all α subtypes has limited our understanding of the physiological roles of different α-AR subtypes, and led to the stagnancy in agonist-based drug development for these receptors. Here we report cryo-EM structures of α-AR in complex with heterotrimeric G-proteins and either the endogenous common agonist epinephrine or the α-AR-specific synthetic agonist A61603. These structures provide molecular insights into the mechanisms underlying the discrimination between α-AR and α-AR by A61603. Guided by the structures and corresponding molecular dynamics simulations, we engineer α-AR mutants that are not responsive to A61603, and α-AR mutants that can be potently activated by A61603. Together, these findings advance our understanding of the agonist specificity for α-ARs at the molecular level, opening the possibility of rational design of subtype-specific agonists.
External linksNat Commun / PubMed:37563160 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.1 Å
Structure data

41267

8thk

EMDB-41267, PDB-8thk:
Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with heterotrimeric Gq-protein
Method: EM (single particle) / Resolution: 2.6 Å

41268

8thl

EMDB-41268, PDB-8thl:
Cryo-EM structure of epinephrine-bound alpha-1A-adrenergic receptor in complex with heterotrimeric Gq-protein
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-CGZ:
N-[(5S)-5-(4,5-dihydro-1H-imidazol-2-yl)-2-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl]methanesulfonamide

ChemComp-ALE:
L-EPINEPHRINE / medication, hormone*YM

Source
  • homo sapiens (human)
  • enterobacteria phage t4 (virus)
KeywordsSIGNALING PROTEIN / Alpha-1A-adrenergic receptor / A61603 / Epinephrine

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