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Structure paper

TitleStructural insights into intron catalysis and dynamics during splicing.
Journal, issue, pagesNature, Vol. 624, Issue 7992, Page 682-688, Year 2023
Publish dateNov 22, 2023
AuthorsLing Xu / Tianshuo Liu / Kevin Chung / Anna Marie Pyle /
PubMed AbstractThe group II intron ribonucleoprotein is an archetypal splicing system with numerous mechanistic parallels to the spliceosome, including excision of lariat introns. Despite the importance of ...The group II intron ribonucleoprotein is an archetypal splicing system with numerous mechanistic parallels to the spliceosome, including excision of lariat introns. Despite the importance of branching in RNA metabolism, structural understanding of this process has remained elusive. Here we present a comprehensive analysis of three single-particle cryogenic electron microscopy structures captured along the splicing pathway. They reveal the network of molecular interactions that specifies the branchpoint adenosine and positions key functional groups to catalyse lariat formation and coordinate exon ligation. The structures also reveal conformational rearrangements of the branch helix and the mechanism of splice site exchange that facilitate the transition from branching to ligation. These findings shed light on the evolution of splicing and highlight the conservation of structural components, catalytic mechanism and dynamical strategies retained through time in premessenger RNA splicing machines.
External linksNature / PubMed:37993708 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.1 Å
Structure data

40985

8t2r

EMDB-40985, PDB-8t2r:
Structure of a group II intron ribonucleoprotein in the pre-ligation (pre-2F) state
Method: EM (single particle) / Resolution: 3.1 Å

40986

8t2s

EMDB-40986, PDB-8t2s:
Structure of a group II intron ribonucleoprotein in the pre-branching (pre-1F) state
Method: EM (single particle) / Resolution: 3.0 Å

40987

8t2t

EMDB-40987, PDB-8t2t:
Structure of a group II intron ribonucleoprotein in the post-ligation (post-2F) state
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NH4:
AMMONIUM ION

ChemComp-MG:
Unknown entry

Source
  • [eubacterium] rectale (bacteria)
KeywordsTransferase/RNA / RNP / RNA / Transferase-RNA complex

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