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TitleCEP104/FAP256 and associated cap complex maintain stability of the ciliary tip.
Journal, issue, pagesJ Cell Biol, Vol. 222, Issue 11, Year 2023
Publish dateNov 6, 2023
AuthorsThibault Legal / Mireya Parra / Maxwell Tong / Corbin S Black / Ewa Joachimiak / Melissa Valente-Paterno / Karl Lechtreck / Jacek Gaertig / Khanh Huy Bui /
PubMed AbstractCilia are essential organelles that protrude from the cell body. Cilia are made of a microtubule-based structure called the axoneme. In most types of cilia, the ciliary tip is distinct from the rest ...Cilia are essential organelles that protrude from the cell body. Cilia are made of a microtubule-based structure called the axoneme. In most types of cilia, the ciliary tip is distinct from the rest of the cilium. Here, we used cryo-electron tomography and subtomogram averaging to obtain the structure of the ciliary tip of the ciliate Tetrahymena thermophila. We show that the microtubules at the tip are highly crosslinked with each other and stabilized by luminal proteins, plugs, and cap proteins at the plus ends. In the tip region, the central pair lacks typical projections and twists significantly. By analyzing cells lacking a ciliary tip-enriched protein CEP104/FAP256 by cryo-electron tomography and proteomics, we discovered candidates for the central pair cap complex and explained the potential functions of CEP104/FAP256. These data provide new insights into the function of the ciliary tip and the mechanisms of ciliary assembly and length regulation.
External linksJ Cell Biol / PubMed:37756660 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution9.0 - 130.0 Å
Structure data

EMDB-40891: Main central pair structure
Method: EM (subtomogram averaging) / Resolution: 22.6 Å

EMDB-40892: Ciliary cap complex from Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 130.0 Å

EMDB-40894: Singlet A-tubule from Tetrahymena thermophila
Method: EM (subtomogram averaging) / Resolution: 15.1 Å

EMDB-40896: Tip central pair consensus map from Tetrahymena thermophila cilia
Method: EM (subtomogram averaging) / Resolution: 12.6 Å

EMDB-40897: C1 refinement of the tip central pair subtomogram average
Method: EM (subtomogram averaging) / Resolution: 9.0 Å

EMDB-40898: C1 MAPS refinement of ciliary tip central pair subtomogram average
Method: EM (subtomogram averaging) / Resolution: 9.4 Å

EMDB-40899: C2 refinement of ciliary tip central pair subtomogram average
Method: EM (subtomogram averaging) / Resolution: 9.1 Å

EMDB-40900: C2 MAPs refinement of ciliary tip central pair subtomogram average
Method: EM (subtomogram averaging) / Resolution: 9.2 Å

EMDB-40909: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (consensus map)
Method: EM (subtomogram averaging) / Resolution: 23.8 Å

EMDB-40910: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (C1 refinement)
Method: EM (subtomogram averaging) / Resolution: 16.7 Å

EMDB-40911: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (C2 refinement)
Method: EM (subtomogram averaging) / Resolution: 18.4 Å

EMDB-40912: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (composite map)
Method: EM (subtomogram averaging) / Resolution: 16.7 Å

EMDB-40913: Ciliary tip tomogram of Tetrahymena thermophila
Method: EM (tomography)

Source
  • Tetrahymena thermophila (eukaryote)

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