EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and functional insights into the T-even type bacteriophage topoisomerase II.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 8719, Year 2024
掲載日	2024年10月8日
著者	Yuhui Xin / Runqi Xian / Yunge Yang / Jingyuan Cong / Zihe Rao / Xuemei Li / Yutao Chen /
PubMed 要旨	T-even type bacteriophages are virulent phages commonly used as model organisms, playing a crucial role in understanding various biological processes. One such process involves the regulation of DNA ...T-even type bacteriophages are virulent phages commonly used as model organisms, playing a crucial role in understanding various biological processes. One such process involves the regulation of DNA topology during phage replication upon host infection, governed by type IIA DNA topoisomerases. In spite of various studies on prokaryotic and eukaryotic counterparts, viral topoisomerase II remains insufficiently understood, especially the unique domain composition of T4 phage. In this study, we determine the cryo-EM structures of topoisomerase II from T4 and T6 phages, including full-length structures of both apo and DNA-binding states which have never been determined before. Together with other conformational states, these structures provide an explicit blueprint of mechanisms of phage topoisomerase II. Particularly, the asymmetric dimeric interactions observed in cryo-EM structures of T6 phage topoisomerase II ATPase domain and central domain bound with DNA shed light on the asynchronous ATP usage and asynchronous cleavage of the G-segment DNA, respectively. The elucidation of phage topoisomerase II's structures and functions not only enhances our understanding of mechanisms and evolutionary parallels with prokaryotic and eukaryotic homologs but also highlights its potential as a model for developing type IIA topoisomerase inhibitors.
リンク	Nat Commun / PubMed:39379365 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.8 - 6.8 Å
構造データ	39391 8ylu EMDB-39391, PDB-8ylu: structure of phage T6 topoisomerase II central domain bound with DNA 手法: EM (単粒子) / 解像度: 2.8 Å 39433 8yo1 EMDB-39433, PDB-8yo1: structure of phage T6 topoisomerase II ATPase domain bound with AMPPNP 手法: EM (単粒子) / 解像度: 3.55 Å 39434 8yo3 EMDB-39434, PDB-8yo3: structure of phage T4 topoisomerase II central domain 手法: EM (単粒子) / 解像度: 3.62 Å 39435 8yo4 EMDB-39435, PDB-8yo4: structure of phage T4 topoisomerase II central domain bound with DNA 手法: EM (単粒子) / 解像度: 3.2 Å 39436 8yo5 EMDB-39436, PDB-8yo5: structure of phage T6 topoisomerase II central domain 手法: EM (単粒子) / 解像度: 3.93 Å 39437 8yo7 EMDB-39437, PDB-8yo7: structure of phage T6 topoisomerase II central domain bound with DNA and m-AMSA 手法: EM (単粒子) / 解像度: 3.16 Å 39438 8yo9 EMDB-39438, PDB-8yo9: structure of phage T4 topoisomerase II gp52 subunit WHD-open state 手法: EM (単粒子) / 解像度: 6.12 Å 39444 8yod EMDB-39444, PDB-8yod: structure of phage T6 apo full-length topoisomerase II 手法: EM (単粒子) / 解像度: 6.8 Å 39454 8yon EMDB-39454, PDB-8yon: structure of phage T6 full-length topoisomerase II bound with DNA 手法: EM (単粒子) / 解像度: 6.73 Å PDB-9imj: Bacteriophage T6 topoisomerase II ATPase domain crystal strcuture 手法: X-RAY DIFFRACTION / 解像度: 2.8 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-ASW: N-[4-(acridin-9-ylamino)-3-methoxyphenyl]methanesulfonamide / 抗腫瘍剤YM ChemComp-HOH: WATER
由来	enterobacteria phage t6 (ファージ) escherichia phage t4 (ファージ) salmonella phage chi (ファージ) dna molecule (その他) Enterobacteria phage T4 (ファージ)
キーワード	ISOMERASE / topoisomerase II / topoisomerase II ATPase domain