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TitleFusion then fission: splitting and reassembly of an artificial fusion-protein nanocage.
Journal, issue, pagesChem Commun (Camb), Vol. 60, Issue 34, Page 4605-4608, Year 2024
Publish dateApr 23, 2024
AuthorsNaoya Ohara / Norifumi Kawakami / Ryoichi Arai / Naruhiko Adachi / Akihito Ikeda / Toshiya Senda / Kenji Miyamoto /
PubMed AbstractA split-protein system is a simple approach to introduce new termini which are useful as modification sites in protein engineering, but has been adapted mainly for monomeric proteins. Here we ...A split-protein system is a simple approach to introduce new termini which are useful as modification sites in protein engineering, but has been adapted mainly for monomeric proteins. Here we demonstrate the design of split subunits of the 60-mer artificial fusion-protein nanocage TIP60. The subunit fragments successfully reformed the cage structure in the same manner as prior to splitting. One of the newly introduced terminals at the interior surface can be modified using a tag peptide and green fluorescent protein. Therefore, the termini could serve as a versatile modification site for incorporating a wide variety of functional peptides and proteins.
External linksChem Commun (Camb) / PubMed:38586927
MethodsEM (single particle)
Resolution7.44 Å
Structure data

EMDB-38931: Cryo-EM structure of artificial protein nanocage mTIP120-Ba
Method: EM (single particle) / Resolution: 7.44 Å

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  • synthetic construct (others)

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