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TitleCryo-EM structure of small-molecule agonist bound delta opioid receptor-G complex enables discovery of biased compound.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 8284, Year 2024
Publish dateSep 27, 2024
AuthorsLin Cheng / Zhuang Miao / Sicen Liu / Zhe Li / Hong Fu / Chanjuan Xu / Shilong Hu / Chang Zhao / Yuxuan Liu / Tiantian Zhao / Wencheng Liu / Heli Wang / Runduo Liu / Wei Yan / Xiangdong Tang / Jianfeng Liu / Zhenhua Shao / Bowen Ke /
PubMed AbstractDelta opioid receptor (δOR) plays a pivotal role in modulating human sensation and emotion. It is an attractive target for drug discovery since, unlike Mu opioid receptor, it is associated with low ...Delta opioid receptor (δOR) plays a pivotal role in modulating human sensation and emotion. It is an attractive target for drug discovery since, unlike Mu opioid receptor, it is associated with low risk of drug dependence. Despite its potential applications, the pharmacological properties of δOR, including the mechanisms of activation by small-molecule agonists and the complex signaling pathways it engages, as well as their relation to the potential side effects, remain poorly understood. In this study, we use cryo-electron microscopy (cryo-EM) to determine the structure of the δOR-G complex when bound to a small-molecule agonist (ADL5859). Moreover, we design a series of probes to examine the key receptor-ligand interaction site and identify a region involved in signaling bias. Using ADL06 as a chemical tool, we elucidate the relationship between the β-arrestin pathway of the δOR and its biological functions, such as analgesic tolerance and convulsion activities. Notably, we discover that the β-arrestin recruitment of δOR might be linked to reduced gastrointestinal motility. These insights enhance our understanding of δOR's structure, signaling pathways, and biological functions, paving the way for the structure-based drug discovery.
External linksNat Commun / PubMed:39333070 / PubMed Central
MethodsEM (single particle)
Resolution3.45 Å
Structure data

38909

8y45

EMDB-38909, PDB-8y45:
Cryo-EM structure of opioid receptor with biased agonist
Method: EM (single particle) / Resolution: 3.45 Å

Chemicals

PDB-1lxy:
Crystal Structure of Arginine Deiminase covalently linked with L-citrulline

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsSIGNALING PROTEIN / STRUCTURAL PROTEIN

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