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TitleCryo-EM structure and functional analysis of the chromatin remodeler RSF.
Journal, issue, pagesActa Crystallogr F Struct Biol Commun, Vol. 80, Issue Pt 6, Page 125-134, Year 2024
Publish dateJun 1, 2024
AuthorsJiale Zhang / Heyu Zhao / Binqian Zou / Huadong Li / Shuqi Dong / Jiali Guan / Chi Wang / Weijie Li / Yutong Liu / Yingying Chen / Nadia Rasheed / Jun He /
PubMed AbstractThe RSF complex belongs to the ISWI chromatin-remodeling family and is composed of two subunits: RSF1 (remodeling and spacing factor 1) and SNF2h (sucrose nonfermenting protein 2 homolog). The RSF ...The RSF complex belongs to the ISWI chromatin-remodeling family and is composed of two subunits: RSF1 (remodeling and spacing factor 1) and SNF2h (sucrose nonfermenting protein 2 homolog). The RSF complex participates in nucleosome spacing and assembly, and subsequently promotes nucleosome maturation. Although SNF2h has been extensively studied in the last few years, the structural and functional properties of the remodeler RSF1 still remain vague. Here, a cryo-EM structure of the RSF-nucleosome complex is reported. The 3D model shows a two-lobe architecture of RSF, and the structure of the RSF-nucleosome (flanked with linker DNA) complex shows that the RSF complex moves the DNA away from the histone octamer surface at the DNA-entry point. Additionally, a nucleosome-sliding assay and a restriction-enzyme accessibility assay show that the RSF1 subunit may cause changes in the chromatin-remodeling properties of SNF2h. As a `nucleosome ruler', the results of an RSF-dinucleosome binding affinity test led to the proposal that the critical distance that RSF `measures' between two nucleosomes is about 24 base pairs.
External linksActa Crystallogr F Struct Biol Commun / PubMed:38818823 / PubMed Central
MethodsEM (single particle)
Resolution4.91 - 7.4 Å
Structure data

EMDB-38860: structure of RSF-147bp NCP complex Class 0
Method: EM (single particle) / Resolution: 6.08 Å

EMDB-38861: Structure of RSF-147bpNCP complex class 2
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-38864: RSF-38N38NCP complex Class0
Method: EM (single particle) / Resolution: 4.91 Å

EMDB-38865: RSF-38N38NCP complex Class 2
Method: EM (single particle) / Resolution: 7.4 Å

Source
  • Heardaxius (crustacean)

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