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TitleType-III-A structure of mycobacteria CRISPR-Csm complexes involving atypical crRNAs.
Journal, issue, pagesInt J Biol Macromol, Vol. 260, Issue Pt 2, Page 129331, Year 2024
Publish dateJan 11, 2024
AuthorsHongtai Zhang / Mingmin Shi / Xiaoli Ma / Mengxi Liu / Nenhan Wang / Qiuhua Lu / Zekai Li / Yanfeng Zhao / Hongshen Zhao / Hong Chen / Huizhi Zhang / Tao Jiang / Songying Ouyang / Yangao Huo / Lijun Bi /
PubMed AbstractTuberculosis (TB), a leading cause of mortality globally, is a chronic infectious disease caused by Mycobacterium tuberculosis that primarily infiltrates the lung. The mature crRNAs in M. ...Tuberculosis (TB), a leading cause of mortality globally, is a chronic infectious disease caused by Mycobacterium tuberculosis that primarily infiltrates the lung. The mature crRNAs in M. tuberculosis transcribed from the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) locus exhibit an atypical structure featured with 5' and 3' repeat tags at both ends of the intact crRNA, in contrast to typical Type-III-A crRNAs that possess 5' repeat tags and partial crRNA sequences. However, this structural peculiarity particularly concerning the specific binding characteristics of the 3' repeat end within the mature crRNA within the Csm complex, has not been comprehensively elucidated. Here, our Mycobacteria CRISPR-Csm complexes structure represents the largest Csm complex reported to date. It incorporates an atypical Type-III-A CRISPR RNA (crRNA) (46 nt) with 5' 8-nt and 3' 4-nt repeat sequences in the stoichiometry of Mycobacteria Csm12345 The PAM-independent single-stranded RNAs (ssRNAs) are the most suitable substrate for the Csm complex. The 3'-repeat end trimming of mature crRNA was not necessary for its cleavage activity in Type-III-A Csm complex. Our work broadens our understanding of the Type-III-A Csm complex and identifies another mature crRNA processing mechanism in the Type-III-A CRISPR-Cas system based on structural biology.
External linksInt J Biol Macromol / PubMed:38218299
MethodsEM (single particle)
Resolution3.1 - 3.73 Å
Structure data

37499

8wfx

EMDB-37499, PDB-8wfx:
Cryo-EM structure of CRISPR-Csm effector complex from Mycobacterium canettii
Method: EM (single particle) / Resolution: 3.73 Å

38066

8x5d

EMDB-38066, PDB-8x5d:
The cryo-EM structure of the Mycobacterium tuberculosis CRISPR-Csm complex
Method: EM (single particle) / Resolution: 3.1 Å

Source
  • mycobacterium canettii (bacteria)
  • mycobacterium tuberculosis (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / Csm effector complex / Mycobacterium canettii / Cryo-EM structure / Csm1 / Csm2 / Csm3 / Csm4 / Csm5 / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex / Mycobacteria CRISPR-Csm complexes

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