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TitleActivity-based metaproteomics driven discovery and enzymological characterization of potential α-galactosidases in the mouse gut microbiome.
Journal, issue, pagesCommun Chem, Vol. 7, Issue 1, Page 184, Year 2024
Publish dateAug 16, 2024
AuthorsJianbing Jiang / Diana Czuchry / Yanxia Ru / Huipai Peng / Junfeng Shen / Teng Wang / Wenjuan Zhao / Weihua Chen / Sen-Fang Sui / Yaowang Li / Nan Li /
PubMed AbstractThe gut microbiota offers an extensive resource of enzymes, but many remain uncharacterized. To distinguish the activities of similar annotated proteins and mine the potentially applicable ones in ...The gut microbiota offers an extensive resource of enzymes, but many remain uncharacterized. To distinguish the activities of similar annotated proteins and mine the potentially applicable ones in the microbiome, we applied an effective Activity-Based Metaproteomics (ABMP) strategy using a specific activity-based probe (ABP) to screen the entire gut microbiome for directly discovering active enzymes and their potential applications, not for exploring host-microbiome interactions. By using an activity-based cyclophellitol aziridine probe specific to α-galactosidases (AGAL), we successfully identified and characterized several gut microbiota enzymes possessing AGAL activities. Cryo-electron microscopy analysis of a newly characterized enzyme (AGLA5) revealed the covalent binding conformations between the AGAL5 active site and the cyclophellitol aziridine ABP, which could provide insights into the enzyme's catalytic mechanism. The four newly characterized AGALs have diverse potential activities, including raffinose family oligosaccharides (RFOs) hydrolysis and enzymatic blood group transformation. Collectively, we present a ABMP platform that facilitates gut microbiota AGALs discovery, biochemical activity annotations and potential industrial or biopharmaceutical applications.
External linksCommun Chem / PubMed:39152233 / PubMed Central
MethodsEM (single particle)
Resolution3.17 - 3.37 Å
Structure data

36790

8k1a

EMDB-36790, PDB-8k1a:
the wild-typed alpha-galactosidase 5
Method: EM (single particle) / Resolution: 3.28 Å

36943

8k7u

EMDB-36943, PDB-8k7u:
the alpha-galactosidase 5 with Cacl2
Method: EM (single particle) / Resolution: 3.37 Å

36944

8k7v

EMDB-36944, PDB-8k7v:
the alpha-galactosidase 5 with inhibitor ABP2
Method: EM (single particle) / Resolution: 3.17 Å

Chemicals


ChemComp, No image

ChemComp-VQX:
Unknown entry

Source
  • blautia pseudococcoides (bacteria)
KeywordsHYDROLASE / alpha-Galactosidase / Cacl2 / inhibitor ABP2

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