EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of the transcription termination factor Rho engagement with transcribing RNA polymerase from .
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 6, Page eade7093, Year 2023
掲載日	2023年2月10日
著者	Yuko Murayama / Haruhiko Ehara / Mari Aoki / Mie Goto / Takeshi Yokoyama / Shun-Ichi Sekine /
PubMed 要旨	Transcription termination is an essential step in transcription by RNA polymerase (RNAP) and crucial for gene regulation. For many bacterial genes, transcription termination is mediated by the ...Transcription termination is an essential step in transcription by RNA polymerase (RNAP) and crucial for gene regulation. For many bacterial genes, transcription termination is mediated by the adenosine triphosphate-dependent RNA translocase/helicase Rho, which causes RNA/DNA dissociation from the RNAP elongation complex (EC). However, the structural basis of the interplay between Rho and RNAP remains obscure. Here, we report the cryo-electron microscopy structure of the RNAP EC engaged with Rho. The Rho hexamer binds RNAP through the carboxyl-terminal domains, which surround the RNA exit site of RNAP, directing the nascent RNA seamlessly from the RNA exit to its central channel. The β-flap tip at the RNA exit is critical for the Rho-dependent RNA release, and its deletion causes an alternative Rho-RNAP binding mode, which is irrelevant to termination. The Rho binding site overlaps with the binding sites of other macromolecules, such as ribosomes, providing a general basis of gene regulation.
リンク	Sci Adv / PubMed:36753546 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 5.8 Å
構造データ	34996 8hsg EMDB-34996, PDB-8hsg: Thermus thermophilus RNA polymerase elongation complex 手法: EM (単粒子) / 解像度: 3.2 Å 34997 8hsh EMDB-34997, PDB-8hsh: Thermus thermophilus RNA polymerase coreenzyme 手法: EM (単粒子) / 解像度: 3.4 Å 34999 8hsj EMDB-34999, PDB-8hsj: Thermus thermophilus transcription termination factor Rho bound with ADP-BeF3 手法: EM (単粒子) / 解像度: 3.6 Å 35000 8hsl EMDB-35000, PDB-8hsl: Thermus thermophilus RNA polymerase bound with an inverted Rho hexamer 手法: EM (単粒子) / 解像度: 5.8 Å 35004 8hsr EMDB-35004, PDB-8hsr: Thermus thermophilus Rho-engaged RNAP elongation complex 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-36744: Thermus thermophilus Rho-engaged RNAP elongation complex; EC part 手法: EM (単粒子) / 解像度: 3.74 Å EMDB-36745: Thermus thermophilus Rho-engaged RNAP elongation complex; Rho part 手法: EM (単粒子) / 解像度: 4.6 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF*: BERYLLIUM TRIFLUORIDE ION
由来	thermus thermophilus hb8 (サーマス・サーモフィルス) dna molecule (デオキシリボ核酸) synthetic construct (人工物)
キーワード	TRANSCRIPTION (転写 (生物学)) / Transcription elongation complex / RNA polymerase (RNAポリメラーゼ) / RNA polymerase coreenzyme / Transcription termination / ATP-dependent RNA/DNA helicase/translocase / transcription termination factor Rho