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TitleStructural snapshots uncover a key phosphorylation motif in GPCRs driving β-arrestin activation.
Journal, issue, pagesMol Cell, Vol. 83, Issue 12, Page 2091-22107.e7, Year 2023
Publish dateJun 15, 2023
AuthorsJagannath Maharana / Parishmita Sarma / Manish K Yadav / Sayantan Saha / Vinay Singh / Shirsha Saha / Mohamed Chami / Ramanuj Banerjee / Arun K Shukla /
PubMed AbstractAgonist-induced GPCR phosphorylation is a key determinant for the binding and activation of β-arrestins (βarrs). However, it is not entirely clear how different GPCRs harboring divergent ...Agonist-induced GPCR phosphorylation is a key determinant for the binding and activation of β-arrestins (βarrs). However, it is not entirely clear how different GPCRs harboring divergent phosphorylation patterns impart converging active conformation on βarrs leading to broadly conserved functional responses such as desensitization, endocytosis, and signaling. Here, we present multiple cryo-EM structures of activated βarrs in complex with distinct phosphorylation patterns derived from the carboxyl terminus of different GPCRs. These structures help identify a P-X-P-P type phosphorylation motif in GPCRs that interacts with a spatially organized K-K-R-R-K-K sequence in the N-domain of βarrs. Sequence analysis of the human GPCRome reveals the presence of this phosphorylation pattern in a large number of receptors, and its contribution in βarr activation is demonstrated by targeted mutagenesis experiments combined with an intrabody-based conformational sensor. Taken together, our findings provide important structural insights into the ability of distinct GPCRs to activate βarrs through a significantly conserved mechanism.
External linksMol Cell / PubMed:37209686 / PubMed Central
MethodsEM (single particle)
Resolution3.26 - 4.8 Å
Structure data

34173

8go8

EMDB-34173, PDB-8go8:
Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1
Method: EM (single particle) / Resolution: 3.41 Å

34175

8goc

EMDB-34175, PDB-8goc:
Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human Vasopressin V2 receptor, V2R
Method: EM (single particle) / Resolution: 4.18 Å

34178

8goo

EMDB-34178, PDB-8goo:
Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1
Method: EM (single particle) / Resolution: 4.4 Å

34188

8gp3

EMDB-34188, PDB-8gp3:
Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C-X-C chemokine receptor type 4, CXCR4
Method: EM (single particle) / Resolution: 4.8 Å

35104

8i0n

EMDB-35104, PDB-8i0n:
Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 (Local refine)
Method: EM (single particle) / Resolution: 3.26 Å

35106

8i0q

EMDB-35106, PDB-8i0q:
Structure of beta-arrestin1 in complex with a phosphopeptide corresponding to the human C-X-C chemokine receptor type 4, CXCR4 (Local refine)
Method: EM (single particle) / Resolution: 4.45 Å

35114

8i0z

EMDB-35114, PDB-8i0z:
Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human C5a anaphylatoxin chemotactic receptor 1, C5aR1 (Local refine)
Method: EM (single particle) / Resolution: 4.33 Å

35115

8i10

EMDB-35115, PDB-8i10:
Structure of beta-arrestin2 in complex with a phosphopeptide corresponding to the human Vasopressin V2 receptor, V2R (Local refine)
Method: EM (single particle) / Resolution: 3.96 Å

Source
  • rattus norvegicus (Norway rat)
  • homo sapiens (human)
  • mus musculus (house mouse)
  • bos taurus (cattle)
KeywordsSIGNALING PROTEIN/IMMUNE SYSTEM / GPCR / Arrestin / SIGNALING PROTEIN / SIGNALING PROTEIN-IMMUNE SYSTEM complex

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