EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection.
ジャーナル・号・ページ	Nature, Vol. 608, Issue 7923, Page 593-602, Year 2022
掲載日	2022年6月17日
著者	Yunlong Cao / Ayijiang Yisimayi / Fanchong Jian / Weiliang Song / Tianhe Xiao / Lei Wang / Shuo Du / Jing Wang / Qianqian Li / Xiaosu Chen / Yuanling Yu / Peng Wang / Zhiying Zhang / Pulan Liu / Ran An / Xiaohua Hao / Yao Wang / Jing Wang / Rui Feng / Haiyan Sun / Lijuan Zhao / Wen Zhang / Dong Zhao / Jiang Zheng / Lingling Yu / Can Li / Na Zhang / Rui Wang / Xiao Niu / Sijie Yang / Xuetao Song / Yangyang Chai / Ye Hu / Yansong Shi / Linlin Zheng / Zhiqiang Li / Qingqing Gu / Fei Shao / Weijin Huang / Ronghua Jin / Zhongyang Shen / Youchun Wang / Xiangxi Wang / Junyu Xiao / Xiaoliang Sunney Xie /
PubMed 要旨	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineages BA.2.12.1, BA.4 and BA.5 exhibit higher transmissibility than the BA.2 lineage. The receptor binding and immune- ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineages BA.2.12.1, BA.4 and BA.5 exhibit higher transmissibility than the BA.2 lineage. The receptor binding and immune-evasion capability of these recently emerged variants require immediate investigation. Here, coupled with structural comparisons of the spike proteins, we show that BA.2.12.1, BA.4 and BA.5 (BA.4 and BA.5 are hereafter referred collectively to as BA.4/BA.5) exhibit similar binding affinities to BA.2 for the angiotensin-converting enzyme 2 (ACE2) receptor. Of note, BA.2.12.1 and BA.4/BA.5 display increased evasion of neutralizing antibodies compared with BA.2 against plasma from triple-vaccinated individuals or from individuals who developed a BA.1 infection after vaccination. To delineate the underlying antibody-evasion mechanism, we determined the escape mutation profiles, epitope distribution and Omicron-neutralization efficiency of 1,640 neutralizing antibodies directed against the receptor-binding domain of the viral spike protein, including 614 antibodies isolated from people who had recovered from BA.1 infection. BA.1 infection after vaccination predominantly recalls humoral immune memory directed against ancestral (hereafter referred to as wild-type (WT)) SARS-CoV-2 spike protein. The resulting elicited antibodies could neutralize both WT SARS-CoV-2 and BA.1 and are enriched on epitopes on spike that do not bind ACE2. However, most of these cross-reactive neutralizing antibodies are evaded by spike mutants L452Q, L452R and F486V. BA.1 infection can also induce new clones of BA.1-specific antibodies that potently neutralize BA.1. Nevertheless, these neutralizing antibodies are largely evaded by BA.2 and BA.4/BA.5 owing to D405N and F486V mutations, and react weakly to pre-Omicron variants, exhibiting narrow neutralization breadths. The therapeutic neutralizing antibodies bebtelovimab and cilgavimab can effectively neutralize BA.2.12.1 and BA.4/BA.5, whereas the S371F, D405N and R408S mutations undermine most broadly sarbecovirus-neutralizing antibodies. Together, our results indicate that Omicron may evolve mutations to evade the humoral immunity elicited by BA.1 infection, suggesting that BA.1-derived vaccine boosters may not achieve broad-spectrum protection against new Omicron variants.
リンク	Nature / PubMed:35714668 / PubMed Central
手法	EM (単粒子)
解像度	3.07 - 3.74 Å
構造データ	32718 7wr8 EMDB-32718, PDB-7wr8: Local CryoEM structure of the SARS-CoV-2 S6P(B.1.1.529) in complex with BD55-3152 Fab 手法: EM (単粒子) / 解像度: 3.5 Å 32732 7wrl EMDB-32732, PDB-7wrl: Local structure of BD55-1239 Fab and SARS-COV2 Omicron RBD complex 手法: EM (単粒子) / 解像度: 3.51 Å 32734 7wro EMDB-32734, PDB-7wro: Local structure of BD55-3372 and delta spike 手法: EM (単粒子) / 解像度: 3.4 Å 32738 7wrz EMDB-32738, PDB-7wrz: Local resolution of BD55-5840 Fab and SARS-COV2 Omicron RBD 手法: EM (単粒子) / 解像度: 3.26 Å 33019 7x6a EMDB-33019, PDB-7x6a: SARS-CoV-2 BA.2 variant spike protein in complex with Fab BD55-5840 手法: EM (単粒子) / 解像度: 3.5 Å 33210 7xiw EMDB-33210, PDB-7xiw: SARS-CoV-2 Omicron BA.2 variant spike (state 1) 手法: EM (単粒子) / 解像度: 3.62 Å 33211 7xix EMDB-33211, PDB-7xix: SARS-CoV-2 Omicron BA.2 variant spike (state 2) 手法: EM (単粒子) / 解像度: 3.25 Å 33212 7xiy EMDB-33212, PDB-7xiy: SARS-CoV-2 Omicron BA.3 variant spike 手法: EM (単粒子) / 解像度: 3.07 Å 33213 7xiz EMDB-33213, PDB-7xiz: SARS-CoV-2 Omicron BA.3 variant spike (local) 手法: EM (単粒子) / 解像度: 3.74 Å 33323 7xnq EMDB-33323, PDB-7xnq: SARS-CoV-2 Omicron BA.4 variant spike 手法: EM (単粒子) / 解像度: 3.52 Å 33324 7xnr EMDB-33324, PDB-7xnr: SARS-CoV-2 Omicron BA.2.13 variant spike 手法: EM (単粒子) / 解像度: 3.49 Å 33325 7xns EMDB-33325, PDB-7xns: SARS-CoV-2 Omicron BA.4 variant spike 手法: EM (単粒子) / 解像度: 3.48 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) sars coronavirus b012 (SARSコロナウイルス) severe acute respiratory syndrome coronavirus (SARS コロナウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / antibody (抗体) / complex / S6P / SARS-COV2 Omicron / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / sars-cov-2 (SARSコロナウイルス2) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / SARS-COV2 Omicron RBD / Omicron (Ο) / Spike-Fab complex / BA.2 (SARSコロナウイルス2-オミクロン株) / spike / BA.3 / BA.4 / BA.2.13 / BA.2.12.1