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TitleA transformation clustering algorithm and its application in polyribosomes structural profiling.
Journal, issue, pagesNucleic Acids Res, Vol. 50, Issue 16, Page 9001-9011, Year 2022
Publish dateSep 9, 2022
AuthorsWenhong Jiang / Jonathan Wagner / Wenjing Du / Juergen Plitzko / Wolfgang Baumeister / Florian Beck / Qiang Guo /
PubMed AbstractImprovements in cryo-electron tomography sample preparation, electron-microscopy instrumentations, and image processing algorithms have advanced the structural analysis of macromolecules in situ. ...Improvements in cryo-electron tomography sample preparation, electron-microscopy instrumentations, and image processing algorithms have advanced the structural analysis of macromolecules in situ. Beyond such analyses of individual macromolecules, the study of their interactions with functionally related neighbors in crowded cellular habitats, i.e. 'molecular sociology', is of fundamental importance in biology. Here we present a NEighboring Molecule TOpology Clustering (NEMO-TOC) algorithm. We optimized this algorithm for the detection and profiling of polyribosomes, which play both constitutive and regulatory roles in gene expression. Our results suggest a model where polysomes are formed by connecting multiple nonstochastic blocks, in which translation is likely synchronized.
External linksNucleic Acids Res / PubMed:35811088 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution12.3 - 42.5 Å
Structure data

EMDB-33018: the neuron ribosome bound to the membrane
Method: EM (subtomogram averaging) / Resolution: 27.9 Å

EMDB-33072: the tomogram of neuron cortical cell
Method: EM (tomography)

EMDB-33073: one tomogram of the neuron cortical cell
Method: EM (tomography)

EMDB-33074: neighboring ribosomes cluster1
Method: EM (subtomogram averaging) / Resolution: 27.36 Å

EMDB-33076: neighboring ribosomes of cluster3
Method: EM (subtomogram averaging) / Resolution: 42.5 Å

EMDB-33077: ribosome translation conformation 1
Method: EM (subtomogram averaging) / Resolution: 12.3 Å

EMDB-33081: ribosome translation conformation 5
Method: EM (subtomogram averaging) / Resolution: 22.1 Å

Source
  • Rattus (rat)

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