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TitleStructure, gating, and pharmacology of human Ca3.3 channel.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 2084, Year 2022
Publish dateApr 19, 2022
AuthorsLingli He / Zhuoya Yu / Ze Geng / Zhuo Huang / Changjiang Zhang / Yanli Dong / Yiwei Gao / Yuhang Wang / Qihao Chen / Le Sun / Xinyue Ma / Bo Huang / Xiaoqun Wang / Yan Zhao /
PubMed AbstractThe low-voltage activated T-type calcium channels regulate cellular excitability and oscillatory behavior of resting membrane potential which trigger many physiological events and have been ...The low-voltage activated T-type calcium channels regulate cellular excitability and oscillatory behavior of resting membrane potential which trigger many physiological events and have been implicated with many diseases. Here, we determine structures of the human T-type Ca3.3 channel, in the absence and presence of antihypertensive drug mibefradil, antispasmodic drug otilonium bromide and antipsychotic drug pimozide. Ca3.3 contains a long bended S6 helix from domain III, with a positive charged region protruding into the cytosol, which is critical for T-type Ca channel activation at low voltage. The drug-bound structures clearly illustrate how these structurally different compounds bind to the same central cavity inside the Ca3.3 channel, but are mediated by significantly distinct interactions between drugs and their surrounding residues. Phospholipid molecules penetrate into the central cavity in various extent to shape the binding pocket and play important roles in stabilizing the inhibitor. These structures elucidate mechanisms of channel gating, drug recognition, and actions, thus pointing the way to developing potent and subtype-specific drug for therapeutic treatments of related disorders.
External linksNat Commun / PubMed:35440630 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.9 Å
Structure data

32584

7wli

EMDB-32584, PDB-7wli:
CryoEM structure of human low-voltage activated T-type calcium channel CaV3.3 (apo)
Method: EM (single particle) / Resolution: 3.3 Å

32585

7wlj

EMDB-32585, PDB-7wlj:
CryoEM structure of human low-voltage activated T-type calcium channel Cav3.3 in complex with mibefradil (MIB)
Method: EM (single particle) / Resolution: 3.9 Å

32586

7wlk

EMDB-32586, PDB-7wlk:
CryoEM structure of human low-voltage activated T-type calcium channel Cav3.3 in complex with Otilonium Bromide(OB)
Method: EM (single particle) / Resolution: 3.6 Å

32587

7wll

EMDB-32587, PDB-7wll:
CryoEM structure of human low-voltage activated T-type calcium channel Cav3.3 in complex with pimozide(PMZ)
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-MWV:
(1S,2S)-2-(2-{[3-(1H-benzimidazol-2-yl)propyl](methyl)amino}ethyl)-6-fluoro-1-(propan-2-yl)-1,2,3,4-tetrahydronaphthalen-2-yl methoxyacetate / channel blocker*YM / Mibefradil

ChemComp-7TB:
2-[diethyl(methyl)-$l^{4}-azanyl]ethyl 4-[(2-octoxyphenyl)carbonylamino]benzoate

ChemComp-1II:
3-[1-[4,4-bis(4-fluorophenyl)butyl]piperidin-4-yl]-1~{H}-benzimidazol-2-one / Pimozide

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / apo state / MIB / OB / CaV3.3 / PMZ-bound

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