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- EMDB-32584: CryoEM structure of human low-voltage activated T-type calcium ch... -

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Basic information

Entry
Database: EMDB / ID: EMD-32584
TitleCryoEM structure of human low-voltage activated T-type calcium channel CaV3.3 (apo)
Map data
Sample
  • Complex: CaV3.3
    • Protein or peptide: Voltage-dependent T-type calcium channel subunit alpha-1I
  • Ligand: CALCIUM IONCalcium
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CHOLESTEROL HEMISUCCINATE
Function / homology
Function and homology information


sleep / high voltage-gated calcium channel activity / NCAM1 interactions / voltage-gated calcium channel complex / calcium ion import across plasma membrane / neuronal action potential / voltage-gated calcium channel activity / Smooth Muscle Contraction / signal transduction / plasma membrane
Similarity search - Function
Voltage-dependent calcium channel, T-type, alpha-1 subunit / Voltage-dependent calcium channel, alpha-1 subunit / Voltage-gated cation channel calcium and sodium / Voltage-dependent channel domain superfamily / Ion transport domain / Ion transport protein
Similarity search - Domain/homology
Voltage-dependent T-type calcium channel subunit alpha-1I
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsHe L / Yu Z / Dong Y / Chen Q / Zhao Y
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of Sciences China
CitationJournal: Nat Commun / Year: 2022
Title: Structure, gating, and pharmacology of human Ca3.3 channel.
Authors: Lingli He / Zhuoya Yu / Ze Geng / Zhuo Huang / Changjiang Zhang / Yanli Dong / Yiwei Gao / Yuhang Wang / Qihao Chen / Le Sun / Xinyue Ma / Bo Huang / Xiaoqun Wang / Yan Zhao /
Abstract: The low-voltage activated T-type calcium channels regulate cellular excitability and oscillatory behavior of resting membrane potential which trigger many physiological events and have been ...The low-voltage activated T-type calcium channels regulate cellular excitability and oscillatory behavior of resting membrane potential which trigger many physiological events and have been implicated with many diseases. Here, we determine structures of the human T-type Ca3.3 channel, in the absence and presence of antihypertensive drug mibefradil, antispasmodic drug otilonium bromide and antipsychotic drug pimozide. Ca3.3 contains a long bended S6 helix from domain III, with a positive charged region protruding into the cytosol, which is critical for T-type Ca channel activation at low voltage. The drug-bound structures clearly illustrate how these structurally different compounds bind to the same central cavity inside the Ca3.3 channel, but are mediated by significantly distinct interactions between drugs and their surrounding residues. Phospholipid molecules penetrate into the central cavity in various extent to shape the binding pocket and play important roles in stabilizing the inhibitor. These structures elucidate mechanisms of channel gating, drug recognition, and actions, thus pointing the way to developing potent and subtype-specific drug for therapeutic treatments of related disorders.
History
DepositionJan 13, 2022-
Header (metadata) releaseMay 4, 2022-
Map releaseMay 4, 2022-
UpdateMay 25, 2022-
Current statusMay 25, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32584.png

Downloads & links

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Map

FileDownload / File: emd_32584.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.35
Minimum - Maximum-2.2029815 - 3.3274238
Average (Standard dev.)-0.00019152345 (±0.0680774)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 266.24 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : CaV3.3

EntireName: CaV3.3
Components
  • Complex: CaV3.3
    • Protein or peptide: Voltage-dependent T-type calcium channel subunit alpha-1I
  • Ligand: CALCIUM IONCalcium
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CHOLESTEROL HEMISUCCINATE

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Supramolecule #1: CaV3.3

SupramoleculeName: CaV3.3 / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human)

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Macromolecule #1: Voltage-dependent T-type calcium channel subunit alpha-1I

MacromoleculeName: Voltage-dependent T-type calcium channel subunit alpha-1I
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 245.373031 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAESASPPSS SAAAPAAEPG VTTEQPGPRS PPSSPPGLEE PLDGADPHVP HPDLAPIAFF CLRQTTSPRN WCIKMVCNPW FECVSMLVI LLNCVTLGMY QPCDDMDCLS DRCKILQVFD DFIFIFFAME MVLKMVALGI FGKKCYLGDT WNRLDFFIVM A GMVEYSLD ...String:
MAESASPPSS SAAAPAAEPG VTTEQPGPRS PPSSPPGLEE PLDGADPHVP HPDLAPIAFF CLRQTTSPRN WCIKMVCNPW FECVSMLVI LLNCVTLGMY QPCDDMDCLS DRCKILQVFD DFIFIFFAME MVLKMVALGI FGKKCYLGDT WNRLDFFIVM A GMVEYSLD LQNINLSAIR TVRVLRPLKA INRVPSMRIL VNLLLDTLPM LGNVLLLCFF VFFIFGIIGV QLWAGLLRNR CF LEENFTI QGDVALPPYY QPEEDDEMPF ICSLSGDNGI MGCHEIPPLK EQGRECCLSK DDVYDFGAGR QDLNASGLCV NWN RYYNVC RTGSANPHKG AINFDNIGYA WIVIFQVITL EGWVEIMYYV MDAHSFYNFI YFILLIIVGS FFMINLCLVV IATQ FSETK QREHRLMLEQ RQRYLSSSTV ASYAEPGDCY EEIFQYVCHI LRKAKRRALG LYQALQSRRQ ALGPEAPAPA KPGPH AKEP RHYHGKTKGQ GDEGRHLGSR HCQTLHGPAS PGNDHSGREL CPQHSPLDAT PHTLVQPIPA TLASDPASCP CCQHED GRR PSGLGSTDSG QEGSGSGSSA GGEDEADGDG ARSSEDGASS ELGKEEEEEE QADGAVWLCG DVWRETRAKL RGIVDSK YF NRGIMMAILV NTVSMGIEHH EQPEELTNIL EICNVVFTSM FALEMILKLA AFGLFDYLRN PYNIFDSIIV IISIWEIV G QADGGLSVLR TFRLLRVLKL VRFMPALRRQ LVVLMKTMDN VATFCMLLML FIFIFSILGM HIFGCKFSLR TDTGDTVPD RKNFDSLLWA IVTVFQILTQ EDWNVVLYNG MASTSPWASL YFVALMTFGN YVLFNLLVAI LVEGFQAEGD ANRSYSDEDQ SSSNIEEFD KLQEGLDSSG DPKLCPIPMT PNGHLDPSLP LGGHLGPAGA AGPAPRLSLQ PDPMLVALGS RKSSVMSLGR M SYDQRSLS SSRSSYYGPW GRSAAWASRR SSWNSLKHKP PSAEHESLLS AERGGGARVC EVAADEGPPR AAPLHTPHAH HI HHGPHLA HRHRHHRRTL SLDNRDSVDL AELVPAVGAH PRAAWRAAGP APGHEDCNGR MPSIAKDVFT KMGDRGDRGE DEE EIDYTL CFRVRKMIDV YKPDWCEVRE DWSVYLFSPE NRFRVLCQTI IAHKLFDYVV LAFIFLNCIT IALERPQIEA GSTE RIFLT VSNYIFTAIF VGEMTLKVVS LGLYFGEQAY LRSSWNVLDG FLVFVSIIDI VVSLASAGGA KILGVLRVLR LLRTL RPLR VISRAPGLKL VVETLISSLK PIGNIVLICC AFFIIFGILG VQLFKGKFYH CLGVDTRNIT NRSDCMAANY RWVHHK YNF DNLGQALMSL FVLASKDGWV NIMYNGLDAV AVDQQPVTNH NPWMLLYFIS FLLIVSFFVL NMFVGVVVEN FHKCRQH QE AEEARRREEK RLRRLEKKRR KAQRLPYYAT YCHTRLLIHS MCTSHYLDIF ITFIICLNVV TMSLEHYNQP TSLETALK Y CNYMFTTVFV LEAVLKLVAF GLRRFFKDRW NQLDLAIVLL SVMGITLEEI EINAALPINP TIIRIMRVLR IARVLKLLK MATGMRALLD TVVQALPQVG NLGLLFMLLF FIYAALGVEL FGKLVCNDEN PCEGMSRHAT FENFGMAFLT LFQVSTGDNW NGIMKDTLR DCTHDERSCL SSLQFVSPLY FVSFVLTAQF VLINVVVAVL MKHLDDSNKE AQEDAEMDAE LELEMAHGLG P GPRLPTGS PGAPGRGPGG AGGGGDTEGG LCRRCYSPAQ ENLWLDSVSL IIKDSLEGEL TIIDNLSGSI FHHYSSPAGC KK CHHDKQE VQLAETEAFS LNSDRSSSIL LGDDLSLEDP TACPPGRKDS KGELDPPEPM RVGDLGECFF PLSSTAVSPD PEN FLCEME EIPFNPVRSW LKHDSSQAPP SPFSPDASSP LLPMPAEFFH PAVSASQKGP EKGTGTGTLP KIALQGSWAS LRSP RVNCT LLRQATGSDT SLDASPSSSA GSLQTTLEDS LTLSDSPRRA LGPPAPAPGP RAGLSPAARR RLSLRGRGLF SLRGL RAHQ RSHSSGGSTS PGCTHHDSMD PSDEEGRGGA GGGGAGSEHS ETLSSLSLTS LFCPPPPPPA PGLTPARKFS STSSLA APG RPHAAALAHG LARSPSWAAD RSKDPPGRAP LPMGLGPLAP PPQPLPGELE PGDAASKRKR

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Macromolecule #2: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Macromolecule #3: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 3 / Number of copies: 2 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

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Macromolecule #4: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 4 / Number of copies: 11 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

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Macromolecule #5: CHOLESTEROL HEMISUCCINATE

MacromoleculeName: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 5 / Number of copies: 6 / Formula: Y01
Molecular weightTheoretical: 486.726 Da
Chemical component information

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 9.6 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 93988

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