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Title8 Å structure of the outer rings of the Xenopus laevis nuclear pore complex obtained by cryo-EM and AI.
Journal, issue, pagesProtein Cell, Vol. 13, Issue 10, Page 760-777, Year 2022
Publish dateJan 11, 2022
AuthorsLinhua Tai / Yun Zhu / He Ren / Xiaojun Huang / Chuanmao Zhang / Fei Sun /
PubMed AbstractThe nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo- ...The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo-electron microscopic (cryo-EM) structure of the outer rings containing nuclear ring (NR) and cytoplasmic ring (CR) from the Xenopus laevis NPC, with local resolutions reaching 4.9 Å. With the aid of AlphaFold2, we managed to build a pseudoatomic model of the outer rings, including the Y complexes and flanking components. In this most comprehensive and accurate model of outer rings to date, the almost complete Y complex structure exhibits much tighter interaction in the hub region. In addition to two copies of Y complexes, each asymmetric subunit in CR contains five copies of Nup358, two copies of the Nup214 complex, two copies of Nup205 and one copy of newly identified Nup93, while that in NR contains one copy of Nup205, one copy of ELYS and one copy of Nup93. These in-depth structural features represent a great advance in understanding the assembly of NPCs.
External linksProtein Cell / PubMed:35015240 / PubMed Central
MethodsEM (single particle)
Resolution7.8 - 8.9 Å
Structure data

31891

7vci

EMDB-31891, PDB-7vci:
Structure of Xenopus laevis NPC nuclear ring asymmetric unit
Method: EM (single particle) / Resolution: 8.1 Å

EMDB-31892:
Cryo-EM structure of the nuclear ring asymmetric unit core of the Xenopus laevis nuclear pore complex
Method: EM (single particle) / Resolution: 7.8 Å

EMDB-31893:
Cryo-EM structure of nuclear ring asymmetric unit region of Xenopus laevis nuclear pore complex
Method: EM (single particle) / Resolution: 8.6 Å

32056

7vop

EMDB-32056, PDB-7vop:
Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring subunit
Method: EM (single particle) / Resolution: 8.7 Å

EMDB-32060:
Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring subunit core region
Method: EM (single particle) / Resolution: 7.9 Å

EMDB-32061:
Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring Nup358 region
Method: EM (single particle) / Resolution: 8.9 Å

Source
  • xenopus laevis (African clawed frog)
KeywordsNUCLEAR PROTEIN / nuclear pore complex / nuclear ring / asymmetric unit / cytoplasmic ring / cryo-EM / Xenopus laevis

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