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TitleGating mechanism and a modulatory niche of human GluN1-GluN2A NMDA receptors.
Journal, issue, pagesNeuron, Vol. 109, Issue 15, Page 2443-22456.e5, Year 2021
Publish dateAug 4, 2021
AuthorsHan Wang / Shiyun Lv / David Stroebel / Jinbao Zhang / Yijie Pan / Xuejing Huang / Xing Zhang / Pierre Paoletti / Shujia Zhu /
PubMed AbstractN-methyl-D-aspartate (NMDA) receptors are glutamate-gated calcium-permeable ion channels that are widely implicated in synaptic transmission and plasticity. Here, we report a gallery of cryo-electron ...N-methyl-D-aspartate (NMDA) receptors are glutamate-gated calcium-permeable ion channels that are widely implicated in synaptic transmission and plasticity. Here, we report a gallery of cryo-electron microscopy (cryo-EM) structures of the human GluN1-GluN2A NMDA receptor at an overall resolution of 4 Å in complex with distinct ligands or modulators. In the full-length context of GluN1-GluN2A receptors, we visualize the competitive antagonists bound to the ligand-binding domains (LBDs) of GluN1 and GluN2A subunits, respectively. We reveal that the binding of positive allosteric modulator shortens the distance between LBDs and the transmembrane domain (TMD), which further stretches the opening of the gate. In addition, we unexpectedly visualize the binding cavity of the "foot-in-the-door" blocker 9-aminoacridine within the LBD-TMD linker region, differing from the conventional "trapping" blocker binding site at the vestibule within the TMD. Our study provides molecular insights into the crosstalk between LBDs and TMD during channel activation, inhibition, and allosteric transition.
External linksNeuron / PubMed:34186027
MethodsEM (single particle)
Resolution3.8 - 4.3 Å
Structure data

31227

7eoq

EMDB-31227, PDB-7eoq:
Structure of the human GluN1/GluN2A NMDA receptor in the glycine/CPP bound state
Method: EM (single particle) / Resolution: 4.1 Å

31228

7eor

EMDB-31228, PDB-7eor:
Structure of the human GluN1/GluN2A NMDA receptor in the glycine/glutamate/GNE-6901 bound state
Method: EM (single particle) / Resolution: 4.0 Å

31229

7eos

EMDB-31229, PDB-7eos:
Structure of the human GluN1/GluN2A NMDA receptor in the glycine/glutamate bound state
Method: EM (single particle) / Resolution: 3.9 Å

31230

7eot

EMDB-31230, PDB-7eot:
Structure of the human GluN1/GluN2A NMDA receptor in the CGP-78608/glutamate bound state
Method: EM (single particle) / Resolution: 3.8 Å

31231

7eou

EMDB-31231, PDB-7eou:
Structure of the human GluN1/GluN2A NMDA receptor in the glycine/glutamate/GNE-6901/9-AA bound state
Method: EM (single particle) / Resolution: 4.3 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-7RC:
(2R)-4-(3-phosphonopropyl)piperazine-2-carboxylic acid

ChemComp-6RM:
7-[(4-fluoranylphenoxy)methyl]-3-[(1~{R},2~{R})-2-(hydroxymethyl)cyclopropyl]-2-methyl-[1,3]thiazolo[3,2-a]pyrimidin-5-one

ChemComp-J86:
[(1S)-1-[[7-bromanyl-2,3-bis(oxidanylidene)-1,4-dihydroquinoxalin-5-yl]methylamino]ethyl]phosphonic acid

ChemComp-AA:
9-AMINOACRIDINE

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / NMDA receptor

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