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Structure paper

TitleAn interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Journal, issue, pagesCell, Vol. 163, Issue 3, Page 607-619, Year 2015
Publish dateOct 22, 2015
AuthorsJohn C Whitney / Dennis Quentin / Shin Sawai / Michele LeRoux / Brittany N Harding / Hannah E Ledvina / Bao Q Tran / Howard Robinson / Young Ah Goo / David R Goodlett / Stefan Raunser / Joseph D Mougous /
PubMed AbstractType VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane ...Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD(+) and NADP(+). Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.
External linksCell / PubMed:26456113 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.401 - 21.5 Å
Structure data

EMDB-3112:
Negative stain EM structure of the Tse6-Tsi6-VgrG1-EF-Tu complex in detergent
Method: EM (single particle) / Resolution: 19.4 Å

EMDB-3113:
Negative stain EM structure of the Tse6-Tsi6-VgrG1-EagT6-EF-Tu complex
Method: EM (single particle) / Resolution: 21.5 Å

PDB-4zuy:
Structure of Tsi6 from Pseudomonas aeruginosa
Method: X-RAY DIFFRACTION / Resolution: 1.952 Å

PDB-4zv0:
Structure of Tse6 in complex with Tsi6
Method: X-RAY DIFFRACTION / Resolution: 1.401 Å

PDB-4zv4:
Structure of Tse6 in complex with EF-Tu
Method: X-RAY DIFFRACTION / Resolution: 3.504 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-IOD:
IODIDE ION

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • Pseudomonas aeruginosa PAO1 (bacteria)
  • Escherichia coli (E. coli)
  • pseudomonas aeruginosa (strain atcc 15692 / pao1 / 1c / prs 101 / lmg 12228) (bacteria)
  • pseudomonas aeruginosa (bacteria)
KeywordsPROTEIN BINDING / 4-helix bundle / bacterial type VI secretion immunity protein / Tse6 immunity protein / T6SS effector-immunity pair / TRANSLATION / T6SS effector / translation elongation factor

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