+Search query
-Structure paper
Title | Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1. |
---|---|
Journal, issue, pages | Structure, Vol. 30, Issue 2, Page 240-251.e4, Year 2022 |
Publish date | Feb 3, 2022 |
Authors | Jun-Tao Zhang / Feng Yang / Kang Du / Wei-Fang Li / Yuxing Chen / Yong-Liang Jiang / Qiong Li / Cong-Zhao Zhou / |
PubMed Abstract | Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near- ...Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-Å-long tail and an icosahedral capsid of 650 Å in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a β-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications. |
External links | Structure / PubMed:34727518 |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 2.671 - 3.96 Å |
Structure data | EMDB-31078, PDB-7eel: EMDB-31079, PDB-7eep: EMDB-31080, PDB-7eeq: PDB-7eea: |
Chemicals | ChemComp-HOH: |
Source |
|
Keywords | VIRAL PROTEIN / Tailspike receptor-binding domain / VIRUS / Major capsid and cement proteins / Portal and adaptor proteins / Needle head proteins and tailspike head-binding proteins |