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TitleStructure and assembly pattern of a freshwater short-tailed cyanophage Pam1.
Journal, issue, pagesStructure, Vol. 30, Issue 2, Page 240-251.e4, Year 2022
Publish dateFeb 3, 2022
AuthorsJun-Tao Zhang / Feng Yang / Kang Du / Wei-Fang Li / Yuxing Chen / Yong-Liang Jiang / Qiong Li / Cong-Zhao Zhou /
PubMed AbstractDespite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near- ...Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-Å-long tail and an icosahedral capsid of 650 Å in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a β-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications.
External linksStructure / PubMed:34727518
MethodsEM (single particle) / X-ray diffraction
Resolution2.671 - 3.96 Å
Structure data

31078

7eel

EMDB-31078, PDB-7eel:
Cyanophage Pam1 capsid asymmetric unit
Method: EM (single particle) / Resolution: 3.26 Å

31079

7eep

EMDB-31079, PDB-7eep:
Cyanophage Pam1 portal-adaptor complex
Method: EM (single particle) / Resolution: 3.75 Å

31080

7eeq

EMDB-31080, PDB-7eeq:
Cyanophage Pam1 tail machine
Method: EM (single particle) / Resolution: 3.96 Å

PDB-7eea:
Cyanophage Pam1 tailspike receptor-binding domain
Method: X-RAY DIFFRACTION / Resolution: 2.671 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • unidentified (others)
KeywordsVIRAL PROTEIN / Tailspike receptor-binding domain / VIRUS / Major capsid and cement proteins / Portal and adaptor proteins / Needle head proteins and tailspike head-binding proteins

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