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TitlePermeant fluorescent probes visualize the activation of SARM1 and uncover an anti-neurodegenerative drug candidate.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateMay 4, 2021
AuthorsWan Hua Li / Ke Huang / Yang Cai / Qian Wen Wang / Wen Jie Zhu / Yun Nan Hou / Sujing Wang / Sheng Cao / Zhi Ying Zhao / Xu Jie Xie / Yang Du / Chi-Sing Lee / Hon Cheung Lee / Hongmin Zhang / Yong Juan Zhao /
PubMed AbstractSARM1 regulates axonal degeneration through its NAD-metabolizing activity and is a drug target for neurodegenerative disorders. We designed and synthesized fluorescent conjugates of styryl derivative ...SARM1 regulates axonal degeneration through its NAD-metabolizing activity and is a drug target for neurodegenerative disorders. We designed and synthesized fluorescent conjugates of styryl derivative with pyridine to serve as substrates of SARM1, which exhibited large red shifts after conversion. With the conjugates, SARM1 activation was visualized in live cells following elevation of endogenous NMN or treatment with a cell-permeant NMN-analog. In neurons, imaging documented mouse SARM1 activation preceded vincristine-induced axonal degeneration by hours. Library screening identified a derivative of nisoldipine (NSDP) as a covalent inhibitor of SARM1 that reacted with the cysteines, especially Cys311 in its ARM domain and blocked its NMN-activation, protecting axons from degeneration. The Cryo-EM structure showed that SARM1 was locked into an inactive conformation by the inhibitor, uncovering a potential neuroprotective mechanism of dihydropyridines.
External linksElife / PubMed:33944777 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

30700

7djt

EMDB-30700, PDB-7djt:
Human SARM1 inhibitory state bounded with inhibitor dHNN
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-H8L:
O3-methyl O5-(2-methylpropyl) 2,6-dimethyl-4-[2-(oxidanylamino)phenyl]pyridine-3,5-dicarboxylate

Source
  • homo sapiens (human)
KeywordsHYDROLASE / NAD(+) hydrolase

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