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TitleStructural insights into transcriptional regulation of human RNA polymerase III.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 2, Page 220-227, Year 2021
Publish dateFeb 8, 2021
AuthorsQianmin Wang / Shaobai Li / Futang Wan / Youwei Xu / Zhenfang Wu / Mi Cao / Pengfei Lan / Ming Lei / Jian Wu /
PubMed AbstractRNA polymerase III (Pol III) synthesizes structured, essential small RNAs, such as transfer RNA, 5S ribosomal RNA and U6 small nuclear RNA. Pol III, the largest nuclear RNA polymerase, is composed of ...RNA polymerase III (Pol III) synthesizes structured, essential small RNAs, such as transfer RNA, 5S ribosomal RNA and U6 small nuclear RNA. Pol III, the largest nuclear RNA polymerase, is composed of a conserved core region and eight constitutive regulatory subunits, but how these factors jointly regulate Pol III transcription remains unclear. Here, we present cryo-EM structures of human Pol III in both apo and elongating states, which unveil both an orchestrated movement during the apo-to-elongating transition and an unexpected apo state in which the RPC7 subunit tail occupies the DNA-RNA-binding cleft of Pol III, suggesting that RPC7 plays important roles in both autoinhibition and transcription initiation. The structures also reveal a proofreading mechanism for the TFIIS-like subunit RPC10, which stably retains its catalytic position in the secondary channel, explaining the high fidelity of Pol III transcription. Our work provides an integrated picture of the mechanism of Pol III transcription regulation.
External linksNat Struct Mol Biol / PubMed:33558766
MethodsEM (single particle)
Resolution2.9 - 3.1 Å
Structure data

30577

7d58

EMDB-30577, PDB-7d58:
cryo-EM structure of human RNA polymerase III in elongating state
Method: EM (single particle) / Resolution: 2.9 Å

30578

7d59

EMDB-30578, PDB-7d59:
cryo-EM structure of human RNA polymerase III in apo state
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • homo sapiens (human)
KeywordsTRANSCRIPTION / RNA Polymerase III

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