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TitleBat and pangolin coronavirus spike glycoprotein structures provide insights into SARS-CoV-2 evolution.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 1607, Year 2021
Publish dateMar 11, 2021
AuthorsShuyuan Zhang / Shuyuan Qiao / Jinfang Yu / Jianwei Zeng / Sisi Shan / Long Tian / Jun Lan / Linqi Zhang / Xinquan Wang /
PubMed AbstractIn recognizing the host cellular receptor and mediating fusion of virus and cell membranes, the spike (S) glycoprotein of coronaviruses is the most critical viral protein for cross-species ...In recognizing the host cellular receptor and mediating fusion of virus and cell membranes, the spike (S) glycoprotein of coronaviruses is the most critical viral protein for cross-species transmission and infection. Here we determined the cryo-EM structures of the spikes from bat (RaTG13) and pangolin (PCoV_GX) coronaviruses, which are closely related to SARS-CoV-2. All three receptor-binding domains (RBDs) of these two spike trimers are in the "down" conformation, indicating they are more prone to adopt the receptor-binding inactive state. However, we found that the PCoV_GX, but not the RaTG13, spike is comparable to the SARS-CoV-2 spike in binding the human ACE2 receptor and supporting pseudovirus cell entry. We further identified critical residues in the RBD underlying different activities of the RaTG13 and PCoV_GX/SARS-CoV-2 spikes. These results collectively indicate that tight RBD-ACE2 binding and efficient RBD conformational sampling are required for the evolution of SARS-CoV-2 to gain highly efficient infection.
External linksNat Commun / PubMed:33707453 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.93 Å
Structure data

30416

7cn4

EMDB-30416, PDB-7cn4:
Cryo-EM structure of bat RaTG13 spike glycoprotein
Method: EM (single particle) / Resolution: 2.93 Å

30418

7cn8

EMDB-30418, PDB-7cn8:
Cryo-EM structure of PCoV_GX spike glycoprotein
Method: EM (single particle) / Resolution: 2.5 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-EIC:
LINOLEIC ACID

Source
  • bat coronavirus ratg13
  • pangolin coronavirus
KeywordsVIRAL PROTEIN / spike

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