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TitleStructural basis for transcription inhibition by E. coli SspA.
Journal, issue, pagesNucleic Acids Res, Vol. 48, Issue 17, Page 9931-9942, Year 2020
Publish dateSep 25, 2020
AuthorsFulin Wang / Jing Shi / Dingwei He / Bei Tong / Chao Zhang / Aijia Wen / Yu Zhang / Yu Feng / Wei Lin /
PubMed AbstractStringent starvation protein A (SspA) is an RNA polymerase (RNAP)-associated protein involved in nucleotide metabolism, acid tolerance and virulence of bacteria. Despite extensive biochemical and ...Stringent starvation protein A (SspA) is an RNA polymerase (RNAP)-associated protein involved in nucleotide metabolism, acid tolerance and virulence of bacteria. Despite extensive biochemical and genetic analyses, the precise regulatory role of SspA in transcription is still unknown, in part, because of a lack of structural information for bacterial RNAP in complex with SspA. Here, we report a 3.68 Å cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo) with SspA. Unexpectedly, the structure reveals that SspA binds to the E. coli σ70-RNAP holoenzyme as a homodimer, interacting with σ70 region 4 and the zinc binding domain of EcoRNAP β' subunit simultaneously. Results from fluorescent polarization assays indicate the specific interactions between SspA and σ70 region 4 confer its σ selectivity, thereby avoiding its interactions with σs or other alternative σ factors. In addition, results from in vitro transcription assays verify that SspA inhibits transcription probably through suppressing promoter escape. Together, the results here provide a foundation for understanding the unique physiological function of SspA in transcription regulation in bacteria.
External linksNucleic Acids Res / PubMed:32785630 / PubMed Central
MethodsEM (single particle)
Resolution3.58 - 3.68 Å
Structure data

30307

7c97

EMDB-30307, PDB-7c97:
Cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo) with SspA
Method: EM (single particle) / Resolution: 3.68 Å

30376

7chw

EMDB-30376: Cryo-EM structure of anEscherichia coli RNAP-promoter open complex (RPo)
PDB-7chw: Cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo)
Method: EM (single particle) / Resolution: 3.58 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
  • escherichia coli (strain k12) (bacteria)
KeywordsTRANSFERASE/DNA / Stringent starvation protein A / RNA polymerase / promoter escape / zinc binding domain / GENE REGULATION / TRANSFERASE-DNA complex / TRANSCRIPTION/DNA / Transcription initiation complex / TRANSCRIPTION / TRANSCRIPTION-DNA complex

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