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TitleCryo-electron tomography of cardiac myofibrils reveals a 3D lattice spring within the Z-discs.
Journal, issue, pagesCommun Biol, Vol. 3, Issue 1, Page 585, Year 2020
Publish dateOct 16, 2020
AuthorsToshiyuki Oda / Haruaki Yanagisawa /
PubMed AbstractThe Z-disc forms a boundary between sarcomeres, which constitute structural and functional units of striated muscle tissue. Actin filaments from adjacent sarcomeres are cross-bridged by α-actinin in ...The Z-disc forms a boundary between sarcomeres, which constitute structural and functional units of striated muscle tissue. Actin filaments from adjacent sarcomeres are cross-bridged by α-actinin in the Z-disc, allowing transmission of tension across the myofibril. Despite decades of studies, the 3D structure of Z-disc has remained elusive due to the limited resolution of conventional electron microscopy. Here, we observed porcine cardiac myofibrils using cryo-electron tomography and reconstructed the 3D structures of the actin-actinin cross-bridging complexes within the Z-discs in relaxed and activated states. We found that the α-actinin dimers showed contraction-dependent swinging and sliding motions in response to a global twist in the F-actin lattice. Our observation suggests that the actin-actinin complex constitutes a molecular lattice spring, which maintains the integrity of the Z-disc during the muscle contraction cycle.
External linksCommun Biol / PubMed:33067529 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution23.0 Å
Structure data

EMDB-30242:
Cardiac Z-disc in EGTA+ATP state, F-actin part (see the additional maps for the composite map and other components)
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-30243:
Cardiac Z-disc in Ca+ATP state, F-actin part (see the additional maps for the composite map and other components)
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

Source
  • Sus scrofa domesticus (domestic pig)

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