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-Structure paper
Title | CryoEM structure of the tegumented capsid of Epstein-Barr virus. |
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Journal, issue, pages | Cell Res, Vol. 30, Issue 10, Page 873-884, Year 2020 |
Publish date | Jul 3, 2020 |
Authors | Zhihai Li / Xiao Zhang / Lili Dong / Jingjing Pang / Miao Xu / Qian Zhong / Mu-Sheng Zeng / Xuekui Yu / |
PubMed Abstract | Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, ...Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, including the icosahedral capsid, the dodecameric portal and the capsid-associated tegument complex (CATC). Our in situ portal from the tegumented capsid adopts a closed conformation with its channel valve holding the terminal viral DNA and with its crown region firmly engaged by three layers of ring-like dsDNA, which, together with the penton flexibility, effectively alleviates the capsid inner pressure placed on the portal cap. In contrast, the CATCs, through binding to the flexible penton vertices in a stoichiometric manner, accurately increase the inner capsid pressure to facilitate the pressure-driven genome delivery. Together, our results provide important insights into the mechanism by which the EBV capsid, portal, packaged genome and the CATCs coordinately achieve a pressure balance to simultaneously benefit both viral genome retention and ejection. |
External links | Cell Res / PubMed:32620850 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.5 - 5.5 Å |
Structure data | EMDB-30146: EMDB-30152: EMDB-30155, PDB-7bqt: EMDB-30156: EMDB-30157, PDB-7bqx: EMDB-30158, PDB-7br7: EMDB-30159, PDB-7br8: EMDB-30162: Epstein-Barr virus, icosahedral tegumented capsid reconstruction |
Source |
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Keywords | VIRAL PROTEIN / Epstein-Barr virus / portal dodecomer / tegumented capsid / portal vertex / CATC / portal-proximal penton vertex / CATC binding / asymmetric reconstruction / C5 penton vertex / CATC absent / VIRUS / icosahedral reconstruction |