EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture of the herpesvirus genome-packaging complex and implications for DNA translocation.
ジャーナル・号・ページ	Protein Cell, Vol. 11, Issue 5, Page 339-351, Year 2020
掲載日	2020年4月23日
著者	Yunxiang Yang / Pan Yang / Nan Wang / Zhonghao Chen / Dan Su / Z Hong Zhou / Zihe Rao / Xiangxi Wang /
PubMed 要旨	Genome packaging is a fundamental process in a viral life cycle and a prime target of antiviral drugs. Herpesviruses use an ATP-driven packaging motor/terminase complex to translocate and cleave ...Genome packaging is a fundamental process in a viral life cycle and a prime target of antiviral drugs. Herpesviruses use an ATP-driven packaging motor/terminase complex to translocate and cleave concatemeric dsDNA into procapsids but its molecular architecture and mechanism are unknown. We report atomic structures of a herpesvirus hexameric terminase complex in both the apo and ADP•BeF3-bound states. Each subunit of the hexameric ring comprises three components-the ATPase/terminase pUL15 and two regulator/fixer proteins, pUL28 and pUL33-unlike bacteriophage terminases. Distal to the nuclease domains, six ATPase domains form a central channel with conserved basic-patches conducive to DNA binding and trans-acting arginine fingers are essential to ATP hydrolysis and sequential DNA translocation. Rearrangement of the nuclease domains mediated by regulatory domains converts DNA translocation mode to cleavage mode. Our structures favor a sequential revolution model for DNA translocation and suggest mechanisms for concerted domain rearrangements leading to DNA cleavage.
リンク	Protein Cell / PubMed:32328903 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 4.5 Å
構造データ	30090 6m5r EMDB-30090, PDB-6m5r: The coordinates of the apo monomeric terminase complex 手法: EM (単粒子) / 解像度: 3.5 Å 30091 6m5s EMDB-30091, PDB-6m5s: The coordinates of the apo hexameric terminase complex 手法: EM (単粒子) / 解像度: 3.9 Å 30092 6m5t EMDB-30092, PDB-6m5t: The coordinate of the nuclease domain of the apo terminase complex 手法: EM (単粒子) / 解像度: 3.6 Å 30093 6m5u EMDB-30093, PDB-6m5u: The coordinates of the monomeric terminase complex in the presence of the ADP-BeF3 手法: EM (単粒子) / 解像度: 3.8 Å 30094 6m5v EMDB-30094, PDB-6m5v: The coordinate of the hexameric terminase complex in the presence of the ADP-BeF3 手法: EM (単粒子) / 解像度: 4.5 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	human alphaherpesvirus 1 strain 17 (ヘルペスウイルス) Human herpesvirus 1 (ヘルペスウイルス) human herpesvirus 1 (strain 17) (ヘルペスウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / HSV-1 (単純ヘルペスウイルス) / terminase complex / apo state / hexamer terminase complex / nuclease domain (ヌクレアーゼ) / apo terminase complex / ADP-BeF3 / monomer (モノマー)