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TitleStructural insights into tetraspanin CD9 function.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 1606, Year 2020
Publish dateMar 30, 2020
AuthorsRie Umeda / Yuhkoh Satouh / Mizuki Takemoto / Yoshiko Nakada-Nakura / Kehong Liu / Takeshi Yokoyama / Mikako Shirouzu / So Iwata / Norimichi Nomura / Ken Sato / Masahito Ikawa / Tomohiro Nishizawa / Osamu Nureki /
PubMed AbstractTetraspanins play critical roles in various physiological processes, ranging from cell adhesion to virus infection. The members of the tetraspanin family have four membrane-spanning domains and short ...Tetraspanins play critical roles in various physiological processes, ranging from cell adhesion to virus infection. The members of the tetraspanin family have four membrane-spanning domains and short and large extracellular loops, and associate with a broad range of other functional proteins to exert cellular functions. Here we report the crystal structure of CD9 and the cryo-electron microscopic structure of CD9 in complex with its single membrane-spanning partner protein, EWI-2. The reversed cone-like molecular shape of CD9 generates membrane curvature in the crystalline lipid layers, which explains the CD9 localization in regions with high membrane curvature and its implications in membrane remodeling. The molecular interaction between CD9 and EWI-2 is mainly mediated through the small residues in the transmembrane region and protein/lipid interactions, whereas the fertilization assay revealed the critical involvement of the LEL region in the sperm-egg fusion, indicating the different dependency of each binding domain for other partner proteins.
External linksNat Commun / PubMed:32231207 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.701 - 8.19 Å
Structure data

30026

6k4j

EMDB-30026: the CD9 and EWI-2 complex
PDB-6k4j: Crystal Structure of the the CD9
Method: EM (single particle) / Resolution: 8.19 Å

30027

6k4j

EMDB-30027: the CD9 and EWI-2 complex attached to an anti-CD9 Fab fragment
PDB-6k4j: Crystal Structure of the the CD9
Method: EM (single particle) / Resolution: 7.3 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-NI:
NICKEL (II) ION

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN

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