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TitleHsp90 provides a platform for kinase dephosphorylation by PP5.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 2197, Year 2023
Publish dateApr 17, 2023
AuthorsMaru Jaime-Garza / Carlos A Nowotny / Daniel Coutandin / Feng Wang / Mariano Tabios / David A Agard /
PubMed AbstractThe Hsp90 molecular chaperone collaborates with the phosphorylated Cdc37 cochaperone for the folding and activation of its many client kinases. As with many kinases, the Hsp90 client kinase CRaf is ...The Hsp90 molecular chaperone collaborates with the phosphorylated Cdc37 cochaperone for the folding and activation of its many client kinases. As with many kinases, the Hsp90 client kinase CRaf is activated by phosphorylation at specific regulatory sites. The cochaperone phosphatase PP5 dephosphorylates CRaf and Cdc37 in an Hsp90-dependent manner. Although dephosphorylating Cdc37 has been proposed as a mechanism for releasing Hsp90-bound kinases, here we show that Hsp90 bound kinases sterically inhibit Cdc37 dephosphorylation indicating kinase release must occur before Cdc37 dephosphorylation. Our cryo-EM structure of PP5 in complex with Hsp90:Cdc37:CRaf reveals how Hsp90 both activates PP5 and scaffolds its association with the bound CRaf to dephosphorylate phosphorylation sites neighboring the kinase domain. Thus, we directly show how Hsp90's role in maintaining protein homeostasis goes beyond folding and activation to include post translationally modifying its client kinases.
External linksNat Commun / PubMed:37069154 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.0 Å
Structure data

29895

8gae

EMDB-29895, PDB-8gae:
Hsp90 provides platform for CRaf dephosphorylation by PP5
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29949: Hsp90:Cdc37:CRaf complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-29957: PP5 TPR domain bound to Hsp90:Cdc37:CRaf complex
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-29973: PP5 bound to Hsp90:Cdc37:CRaf complex
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-29976: PP5 bound to Hsp90:Cdc37:CRaf complex (conformation II)
Method: EM (single particle) / Resolution: 3.8 Å

29984

8gft

EMDB-29984, PDB-8gft:
Hsp90 provides platform for CRaf dephosphorylation by PP5
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsCHAPERONE / kinase / phosphatase / complex

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