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-Structure paper
Title | HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation. |
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Journal, issue, pages | J Mol Biol, Vol. 435, Issue 15, Page 168143, Year 2023 |
Publish date | Aug 1, 2023 |
Authors | Nathaniel Talledge / Huixin Yang / Ke Shi / Raffaele Coray / Guichuan Yu / William G Arndt / Shuyu Meng / Gloria C Baxter / Luiza M Mendonça / Daniel Castaño-Díez / Hideki Aihara / Louis M Mansky / Wei Zhang / |
PubMed Abstract | Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus ...Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used the single particle cryo-electron microscopy method to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 Å resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag lattice structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 Å resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation. |
External links | J Mol Biol / PubMed:37150290 / PubMed Central |
Methods | EM (tomography) / EM (single particle) / X-ray diffraction |
Resolution | 1.98 - 9.1 Å |
Structure data | EMDB-29606: CryoET of HIV-2 Immature Particles EMDB-29607, PDB-8fzc: PDB-7tv2: |
Chemicals | ChemComp-HOH: |
Source |
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Keywords | STRUCTURAL PROTEIN / viral protein / VIRUS LIKE PARTICLE / Retrovirus / Lentivirus / Gag / Capsid / HIV-2 / lattice |