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TitleSen1 architecture: RNA-DNA hybrid resolution, autoregulation, and insights into SETX inactivation in AOA2.
Journal, issue, pagesMol Cell, Vol. 83, Issue 20, Page 3692-33706.e5, Year 2023
Publish dateOct 19, 2023
AuthorsC Denise Appel / Oya Bermek / Venkata P Dandey / Makayla Wood / Elizabeth Viverette / Jason G Williams / Jonathan Bouvette / Amanda A Riccio / Juno M Krahn / Mario J Borgnia / R Scott Williams /
PubMed AbstractThe senataxin (SETX, Sen1 in yeasts) RNA-DNA hybrid resolving helicase regulates multiple nuclear transactions, including DNA replication, transcription, and DNA repair, but the molecular basis for ...The senataxin (SETX, Sen1 in yeasts) RNA-DNA hybrid resolving helicase regulates multiple nuclear transactions, including DNA replication, transcription, and DNA repair, but the molecular basis for Sen1 activities is ill defined. Here, Sen1 cryoelectron microscopy (cryo-EM) reconstructions reveal an elongated inchworm-like architecture. Sen1 is composed of an amino terminal helical repeat Sen1 N-terminal (Sen1N) regulatory domain that is flexibly linked to its C-terminal SF1B helicase motor core (Sen1) via an intrinsically disordered tether. In an autoinhibited state, the Sen1 domain regulates substrate engagement by promoting occlusion of the RNA substrate-binding cleft. The X-ray structure of an activated Sen1 engaging single-stranded RNA and ADP-SO shows that the enzyme encircles RNA and implicates a single-nucleotide power stroke in the Sen1 RNA translocation mechanism. Together, our data unveil dynamic protein-protein and protein-RNA interfaces underpinning helicase regulation and inactivation of human SETX activity by RNA-binding-deficient mutants in ataxia with oculomotor apraxia 2 neurodegenerative disease.
External linksMol Cell / PubMed:37832548 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.01 - 4.56 Å
Structure data

29426

8fth

EMDB-29426, PDB-8fth:
Chaetomium thermophilum SETX - NPPC internal deletion
Method: EM (single particle) / Resolution: 3.17 Å

29439

8ftk

EMDB-29439, PDB-8ftk:
Chaetomium thermophilum SETX (Full-length)
Method: EM (single particle) / Resolution: 4.56 Å

EMDB-29440: SETX-Sen1N domain
Method: EM (single particle) / Resolution: 4.49 Å

PDB-8ftm:
Setx-ssRNA-ADP-SO4 complex
Method: X-RAY DIFFRACTION / Resolution: 3.01 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

Source
  • chaetomium thermophilum (fungus)
  • thermochaetoides thermophila dsm 1495 (fungus)
  • Thermochaetoides thermophila (fungus)
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION / Helicase / DNA repair / RNA-DNA hybrid / TRANSCRIPTION/RNA / TRANSCRIPTION-RNA complex

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