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Structure paper

TitleThe structure of the dynactin complex and its interaction with dynein.
Journal, issue, pagesScience, Vol. 347, Issue 6229, Page 1441-1446, Year 2015
Publish dateMar 27, 2015
AuthorsLinas Urnavicius / Kai Zhang / Aristides G Diamant / Carina Motz / Max A Schlager / Minmin Yu / Nisha A Patel / Carol V Robinson / Andrew P Carter /
PubMed AbstractDynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our ...Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how dynactin is built around a filament containing eight copies of the actin-related protein Arp1 and one of β-actin. The filament is capped at each end by distinct protein complexes, and its length is defined by elongated peptides that emerge from the α-helical shoulder domain. A further 8.2 angstrom structure of the complex between dynein, dynactin, and the motility-inducing cargo adaptor Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and dynactin to stabilize the mutually dependent interactions between all three components.
External linksScience / PubMed:25814576 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.5 - 12.5 Å
Structure data

EMDB-2854:
CryoEM structure of dynactin complex at 6.3 angstrom resolution
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-2855:
CryoEM structure of dynactin complex with p150-glued projection
Method: EM (single particle) / Resolution: 8.6 Å

2856

5adx

EMDB-2856: CryoEM structure of dynactin complex at 4.1 angstrom resolution
PDB-5adx: CryoEM structure of dynactin complex at 4.0 angstrom resolution
Method: EM (single particle) / Resolution: 4.0 Å

2857

5adx

EMDB-2857: CryoEM reconstruction of dynactin complex from pig brain at 4.0 Angstrom resolution overall
PDB-5adx: CryoEM structure of dynactin complex at 4.0 angstrom resolution
Method: EM (single particle) / Resolution: 3.5 Å

2860

5afu

EMDB-2860: Electron cryo-microscopy of dynein/dynactin/GFP-BICD2N complex
PDB-5afu: Cryo-EM structure of dynein tail-dynactin-BICD2N complex
Method: EM (single particle) / Resolution: 8.2 Å

EMDB-2861:
Electron cryo-microscopy of dynein/dynactin/GFP-BICD2N complex
Method: EM (single particle) / Resolution: 11.8 Å

EMDB-2862:
Electron cryo-microscopy of dynein/dynactin/BICD2N complex
Method: EM (single particle) / Resolution: 12.5 Å

PDB-5afr:
N-terminal fragment of dynein heavy chain
Method: X-RAY DIFFRACTION / Resolution: 5 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • Sus scrofa domesticus (domestic pig)
  • Homo sapiens (human)
  • Mus musculus (house mouse)
  • sus scrofa (pig)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsSTRUCTURAL PROTEIN / DYNEIN CO-FACTOR / ACTIN-LIKE FILAMENT / CELLULAR CARGO TRANSPORT / MOTOR PROTEIN / DYNEIN / TAIL / HEAVY CHAIN / DYNACTIN / BICD2 / MOTOR / TRANSPORT

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