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TitleStructural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1.
Journal, issue, pagesElife, Vol. 4, Year 2015
Publish dateAug 10, 2015
AuthorsAgata Butryn / Jan M Schuller / Gabriele Stoehr / Petra Runge-Wollmann / Friedrich Förster / David T Auble / Karl-Peter Hopfner /
PubMed AbstractSwi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent ...Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box-binding protein (TBP):DNA complexes, offering a useful system to address the structural mechanisms of Swi2/Snf2 ATPases. Here, we report the crystal structure of the N-terminal domain of Mot1 in complex with TBP, DNA, and the transcription regulator negative cofactor 2 (NC2). Our data show that Mot1 reduces DNA:NC2 interactions and unbends DNA as compared to the TBP:DNA:NC2 state, suggesting that Mot1 primes TBP:NC2 displacement in an ATP-independent manner. Electron microscopy and cross-linking data suggest that the Swi2/Snf2 domain of Mot1 associates with the upstream DNA and the histone fold of NC2, thereby revealing parallels to some nucleosome remodelers. This study provides a structural framework for how a Swi2/Snf2 ATPase interacts with its substrate DNA:protein complex.
External linksElife / PubMed:26258880 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.78 - 22.0 Å
Structure data

EMDB-2828:
Structure of Mot1 in complex with TBP, NC2 and DNA
Method: EM (single particle) / Resolution: 22.0 Å

PDB-4wzs:
Crystal structure of the Mot1 N-terminal domain in complex with TBP and NC2 bound to a promoter DNA fragment
Method: X-RAY DIFFRACTION / Resolution: 3.78 Å

Source
  • encephalitozoon cuniculi (fungus)
  • encephalitozoon cuniculi (strain gb-m1) (fungus)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / protein-DNA complex / Swi2/Snf2

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