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| Title | Cryo-electron tomography with mixed-scale dense neural networks reveals key steps in deployment of invasion machinery. |
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| Journal, issue, pages | PNAS Nexus, Vol. 1, Issue 4, Page pgac183, Year 2022 |
| Publish date | Sep 4, 2022 |
 Authors | Li-Av Segev-Zarko / Peter D Dahlberg / Stella Y Sun / Daniël M Pelt / Chi Yong Kim / Elizabeth S Egan / James A Sethian / Wah Chiu / John C Boothroyd /   |
| PubMed Abstract | Host cell invasion by intracellular, eukaryotic parasites within the phylum Apicomplexa is a remarkable and active process involving the coordinated action of apical organelles and other structures. ...Host cell invasion by intracellular, eukaryotic parasites within the phylum Apicomplexa is a remarkable and active process involving the coordinated action of apical organelles and other structures. To date, capturing how these structures interact during invasion has been difficult to observe in detail. Here, we used cryogenic electron tomography to image the apical complex of tachyzoites under conditions that mimic resting parasites and those primed to invade through stimulation with calcium ionophore. Through the application of mixed-scale dense networks for image processing, we developed a highly efficient pipeline for annotation of tomograms, enabling us to identify and extract densities of relevant subcellular organelles and accurately analyze features in 3-D. The results reveal a dramatic change in the shape of the anteriorly located apical vesicle upon its apparent fusion with a rhoptry that occurs only in the stimulated parasites. We also present information indicating that this vesicle originates from the vesicles that parallel the intraconoidal microtubules and that the latter two structures are linked by a novel tether. We show that a rosette structure previously proposed to be involved in rhoptry secretion is associated with apical vesicles beyond just the most anterior one. This result, suggesting multiple vesicles are primed to enable rhoptry secretion, may shed light on the mechanisms employs to enable repeated invasion attempts. Using the same approach, we examine merozoites and show that they too possess an apical vesicle just beneath a rosette, demonstrating evolutionary conservation of this overall subcellular organization. |
 External links | PNAS Nexus / PubMed:36329726 / PubMed Central |
| Methods | EM (tomography) |
| Structure data |  EMDB-28139: Toxoplasma gondii apical complex (ionophore stimulated)  EMDB-28140: Toxoplasma gondii apical complex (non-stimulated) |
| Source |
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Toxoplasma gondii (eukaryote)