EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into assembly and function of GluN1-2C, GluN1-2A-2C, and GluN1-2D NMDARs.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 23, Page 4548-44563.e4, Year 2022
掲載日	2022年12月1日
著者	Tsung-Han Chou / Hyunook Kang / Noriko Simorowski / Stephen F Traynelis / Hiro Furukawa /
PubMed 要旨	Neurotransmission mediated by diverse subtypes of N-methyl-D-aspartate receptors (NMDARs) is fundamental for basic brain functions and development as well as neuropsychiatric diseases and disorders. ...Neurotransmission mediated by diverse subtypes of N-methyl-D-aspartate receptors (NMDARs) is fundamental for basic brain functions and development as well as neuropsychiatric diseases and disorders. NMDARs are glycine- and glutamate-gated ion channels that exist as heterotetramers composed of obligatory GluN1 and GluN2(A-D) and/or GluN3(A-B). The GluN2C and GluN2D subunits form ion channels with distinct properties and spatio-temporal expression patterns. Here, we provide the structures of the agonist-bound human GluN1-2C NMDAR in the presence and absence of the GluN2C-selective positive allosteric potentiator (PAM), PYD-106, the agonist-bound GluN1-2A-2C tri-heteromeric NMDAR, and agonist-bound GluN1-2D NMDARs by single-particle electron cryomicroscopy. Our analysis shows unique inter-subunit and domain arrangements of the GluN2C NMDARs, which contribute to functional regulation and formation of the PAM binding pocket and is distinct from GluN2D NMDARs. Our findings here provide the fundamental blueprint to study GluN2C- and GluN2D-containing NMDARs, which are uniquely involved in neuropsychiatric disorders.
リンク	Mol Cell / PubMed:36309015 / PubMed Central
手法	EM (単粒子)
解像度	3.26 - 4.24 Å
構造データ	27953 8e92 EMDB-27953, PDB-8e92: D-cycloserine and glutamate bound Human GluN1a-GluN2C NMDA receptor in intact conformation 手法: EM (単粒子) / 解像度: 3.96 Å 27954 8e93 EMDB-27954, PDB-8e93: D-cycloserine and glutamate bound Human GluN1a-GluN2C NMDA receptor in splayed conformation 手法: EM (単粒子) / 解像度: 3.71 Å 27955 8e94 EMDB-27955, PDB-8e94: PYD-106-bound Human GluN1a-GluN2C NMDA receptor in intact conformation 手法: EM (単粒子) / 解像度: 3.72 Å 27957 8e96 EMDB-27957, PDB-8e96: Glycine and glutamate bound Human GluN1a-GluN2D NMDA receptor 手法: EM (単粒子) / 解像度: 3.38 Å 27958 8e97 EMDB-27958, PDB-8e97: PYD-106-bound Human GluN1a-GluN2C NMDA receptor in splayed conformation 手法: EM (単粒子) / 解像度: 4.19 Å 27959 8e98 EMDB-27959, PDB-8e98: D-cycloserine and glutamate bound Human GluN1a-GluN2C NMDA receptor in nanodisc - intact conformation 手法: EM (単粒子) / 解像度: 3.75 Å EMDB-27960: D-cycloserine and glutamate bound Human GluN1a-GluN2C NMDA receptor in nanodisc - splayed conformation 手法: EM (単粒子) / 解像度: 3.28 Å 27961 8e99 EMDB-27961, PDB-8e99: Human GluN1a-GluN2A-GluN2C triheteromeric NMDA receptor in complex with Nb-4 手法: EM (単粒子) / 解像度: 4.24 Å EMDB-28051: Local refined map of the LBD region of Human GluN1a-GluN2D NMDA receptor 手法: EM (単粒子) / 解像度: 3.26 Å EMDB-28052: Local refined map of ATD region of GluN1a-2D complex 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-28053: Local refined map of the TMD region of Human GluN1a-GluN2D NMDA receptor 手法: EM (単粒子) / 解像度: 3.92 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-IWB: methyl 4-[(2~{R})-3-ethanoyl-1-[2-(2-methyl-1~{H}-indol-3-yl)ethyl]-4-oxidanyl-5-oxidanylidene-2~{H}-pyrrol-2-yl]benzoate ChemComp-GLY: GLYCINE / グリシン / グリシン ChemComp-GLU: GLUTAMIC ACID / グルタミン酸 / グルタミン酸
由来	homo sapiens (ヒト) lama glama (ラマ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Ligand-gated ion channel (リガンド依存性イオンチャネル) / ionotropic glutamate receptor / synaptic protein / voltage-gate ion channel / TRANSPORT PROTEIN/IMMUNE SYSTEM / TRANSPORT PROTEIN-IMMUNE SYSTEM complex