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TitleCompact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 3388, Year 2022
Publish dateJun 13, 2022
AuthorsRitwika S Basu / Michael B Sherman / Matthieu G Gagnon /
PubMed AbstractDuring translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-tRNA) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit joining IF2 ...During translation initiation, initiation factor 2 (IF2) holds initiator transfer RNA (fMet-tRNA) in a specific orientation in the peptidyl (P) site of the ribosome. Upon subunit joining IF2 hydrolyzes GTP and, concomitant with inorganic phosphate (P) release, changes conformation facilitating fMet-tRNA accommodation into the P site and transition of the 70 S ribosome initiation complex (70S-IC) to an elongation-competent ribosome. The mechanism by which IF2 separates from initiator tRNA at the end of translation initiation remains elusive. Here, we report cryo-electron microscopy (cryo-EM) structures of the 70S-IC from Pseudomonas aeruginosa bound to compact IF2-GDP and initiator tRNA. Relative to GTP-bound IF2, rotation of the switch 2 α-helix in the G-domain bound to GDP unlocks a cascade of large-domain movements in IF2 that propagate to the distal tRNA-binding domain C2. The C2-domain relocates 35 angstroms away from tRNA, explaining how IF2 makes way for fMet-tRNA accommodation into the P site. Our findings provide the basis by which IF2 gates the ribosome to the elongation phase.
External linksNat Commun / PubMed:35697706 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

26553

7uiu

EMDB-26553, PDB-7uiu:
N2 sub-domain of IF2 bound to the 30S subunit in the Pseudomonas aeruginosa 70S ribosome initiation complex (focused classification and refinement)
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • pseudomonas aeruginosa pao1 (bacteria)
KeywordsRIBOSOME / Initiation Factor 2 / 70S ribosome / cryo-EM / translation initiation / initiator tRNA / conformational changes

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