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TitleIgG-like bispecific antibodies with potent and synergistic neutralization against circulating SARS-CoV-2 variants of concern.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 5814, Year 2022
Publish dateOct 3, 2022
AuthorsMatthew R Chang / Luke Tomasovic / Natalia A Kuzmina / Adam J Ronk / Patrick O Byrne / Rebecca Johnson / Nadia Storm / Eduardo Olmedillas / Yixuan J Hou / Alexandra Schäfer / Sarah R Leist / Longping V Tse / Hanzhong Ke / Christian Coherd / Katrina Nguyen / Maliwan Kamkaew / Anna Honko / Quan Zhu / Galit Alter / Erica Ollmann Saphire / Jason S McLellan / Anthony Griffiths / Ralph S Baric / Alexander Bukreyev / Wayne A Marasco /
PubMed AbstractMonoclonal antibodies are a promising approach to treat COVID-19, however the emergence of SARS-CoV-2 variants has challenged the efficacy and future of these therapies. Antibody cocktails are being ...Monoclonal antibodies are a promising approach to treat COVID-19, however the emergence of SARS-CoV-2 variants has challenged the efficacy and future of these therapies. Antibody cocktails are being employed to mitigate these challenges, but neutralization escape remains a major challenge and alternative strategies are needed. Here we present two anti-SARS-CoV-2 spike binding antibodies, one Class 1 and one Class 4, selected from our non-immune human single-chain variable fragment (scFv) phage library, that are engineered into four, fully-human IgG-like bispecific antibodies (BsAb). Prophylaxis of hACE2 mice and post-infection treatment of golden hamsters demonstrates the efficacy of the monospecific antibodies against the original Wuhan strain, while promising in vitro results with the BsAbs demonstrate enhanced binding and distinct synergistic effects on neutralizing activity against circulating variants of concern. In particular, one BsAb engineered in a tandem scFv-Fc configuration shows synergistic neutralization activity against several variants of concern including B.1.617.2. This work provides evidence that synergistic neutralization can be achieved using a BsAb scaffold, and serves as a foundation for the future development of broadly reactive BsAbs against emerging variants of concern.
External linksNat Commun / PubMed:36192374 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 4.6 Å
Structure data

EMDB-25618: SARS-CoV-2 VFLIP spike boung to 2 Ab12 Fab fragments
Method: EM (single particle) / Resolution: 4.6 Å

25663

7t3m

EMDB-25663, PDB-7t3m:
SARS-CoV-2 S (Spike Glycoprotein) D614G with Three (3) RBDs Up, Bound to Antibody 2-7 scFv, composite map
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-25689: SARS-CoV-2 S (Spike Glycoprotein) D614G with Three (3) RBDs Up, Bound to Antibody 2-7 scFv, global map with poorly-resolved RBDs and scFvs
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-25690: SARS-CoV-2 S (Spike Glycoprotein) D614G with Three (3) RBDs Up, Bound to Antibody 2-7 scFv, local refinement map
Method: EM (single particle) / Resolution: 3.4 Å

25711

7t67

EMDB-25711, PDB-7t67:
SARS-CoV-2 S (Spike Glycoprotein) D614G with One(1) RBD Up
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • Cell-free gateway cloning vector C-term 8xHis pCellFree_G02 (others)
  • severe acute respiratory syndrome coronavirus 2
  • escherichia coli (E. coli)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / virus / coronavirus / SARS CoV-2 / SARS-CoV-2 / spike / 2-7 / D614G / RBD / 3 RBDs up / three RBDs up / scFv / complex / phage display / VIRAL PROTEIN / local refinement / focused refinement / VIRAL PROTEIN-IMMUNE SYSTEM complex / 1 RBD up / one RBD up

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