Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 29, Issue 8, Page 820-830, Year 2022
Publish date	Jul 18, 2022
Authors	Michelle Y Fry / Vladimíra Najdrová / Ailiena O Maggiolo / Shyam M Saladi / Pavel Doležal / William M Clemons /
PubMed Abstract	Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) ...Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
External links	Nat Struct Mol Biol / PubMed:35851188
Methods	EM (single particle) / X-ray diffraction
Resolution	2.23 - 8.46 Å
Structure data	25373 7sq0 EMDB-25373: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 - nucleotide binding domain map PDB-7sq0: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 Method: EM (single particle) / Resolution: 3.7 Å EMDB-25374: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 - overall map Method: EM (single particle) / Resolution: 4.2 Å EMDB-25375: Nucleotide-free Get3 in the closed form Method: EM (single particle) / Resolution: 8.46 Å PDB-7spy: Get3 bound to ATP from G. intestinalis in the closed form Method: X-RAY DIFFRACTION / Resolution: 2.23 Å PDB-7spz: Nucleotide-free Get3 in two open forms Method: X-RAY DIFFRACTION / Resolution: 3.0 Å
Chemicals	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-SO4: SULFATE ION ChemComp-HOH: WATER ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM
Source	saccharomyces cerevisiae (brewer's yeast) giardia intestinalis (strain atcc 50803 / wb clone c6) (eukaryote) giardia lamblia atcc 50803 (eukaryote)
Keywords	CHAPERONE / Tail-anchored membrane protein targeting Deviant Walker A ATPase targeting factor