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TitleThe role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation.
Journal, issue, pagesElife, Vol. 2, Page e01085, Year 2013
Publish dateNov 28, 2013
AuthorsYashar Sadian / Christos Gatsogiannis / Csilla Patasi / Oliver Hofnagel / Roger S Goody / Marian Farkasovský / Stefan Raunser /
PubMed AbstractSeptins are guanine nucleotide-binding proteins that polymerize into filamentous and higher-order structures. Cdc42 and its effector Gic1 are involved in septin recruitment, ring formation and ...Septins are guanine nucleotide-binding proteins that polymerize into filamentous and higher-order structures. Cdc42 and its effector Gic1 are involved in septin recruitment, ring formation and dissociation. The regulatory mechanisms behind these processes are not well understood. Here, we have used electron microscopy and cryo electron tomography to elucidate the structural basis of the Gic1-septin and Gic1-Cdc42-septin interaction. We show that Gic1 acts as a scaffolding protein for septin filaments forming long and flexible filament cables. Cdc42 in its GTP-form binds to Gic1, which ultimately leads to the dissociation of Gic1 from the filament cables. Surprisingly, Cdc42-GDP is not inactive, but in the absence of Gic1 directly interacts with septin filaments resulting in their disassembly. We suggest that this unanticipated dual function of Cdc42 is crucial for the cell cycle. Based on our results we propose a novel regulatory mechanism for septin filament formation and dissociation. DOI: http://dx.doi.org/10.7554/eLife.01085.001.
External linksElife / PubMed:24286829 / PubMed Central
MethodsEM (tomography)
Resolution60.0 Å
Structure data

EMDB-2504:
Septin-Gic1-Cdc42-GppNHp complex in a single-tilt-axis subtomogram
Method: EM (tomography) / Resolution: 60.0 Å

EMDB-2505:
Septin-Gic1 complex in a single-tilt-axis subtomogram.
Method: EM (tomography) / Resolution: 60.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • Homo sapiens (human)

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