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-Structure paper
Title | Asymmetric localization of the cell division machinery during sporulation. |
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Journal, issue, pages | Elife, Vol. 10, Year 2021 |
Publish date | May 21, 2021 |
Authors | Kanika Khanna / Javier Lopez-Garrido / Joseph Sugie / Kit Pogliano / Elizabeth Villa / |
PubMed Abstract | The Gram-positive bacterium can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the ...The Gram-positive bacterium can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the division septum is formed closer to one pole to yield a smaller forespore and a larger mother cell. Using cryo-electron tomography, genetics and fluorescence microscopy, we found that the organization of the division machinery is different in the two septa. While FtsAZ filaments, the major orchestrators of bacterial cell division, are present uniformly around the leading edge of the invaginating vegetative septa, they are only present on the mother cell side of the invaginating sporulation septa. We provide evidence suggesting that the different distribution and number of FtsAZ filaments impact septal thickness, causing vegetative septa to be thicker than sporulation septa already during constriction. Finally, we show that a sporulation-specific protein, SpoIIE, regulates asymmetric divisome localization and septal thickness during sporulation. |
External links | Elife / PubMed:34018921 / PubMed Central |
Methods | EM (tomography) |
Structure data | EMDB-23963: EMDB-23964: EMDB-23965: EMDB-23966: EMDB-23967: EMDB-23968: |
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