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TitleAsymmetric localization of the cell division machinery during sporulation.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateMay 21, 2021
AuthorsKanika Khanna / Javier Lopez-Garrido / Joseph Sugie / Kit Pogliano / Elizabeth Villa /
PubMed AbstractThe Gram-positive bacterium can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the ...The Gram-positive bacterium can divide via two modes. During vegetative growth, the division septum is formed at the midcell to produce two equal daughter cells. However, during sporulation, the division septum is formed closer to one pole to yield a smaller forespore and a larger mother cell. Using cryo-electron tomography, genetics and fluorescence microscopy, we found that the organization of the division machinery is different in the two septa. While FtsAZ filaments, the major orchestrators of bacterial cell division, are present uniformly around the leading edge of the invaginating vegetative septa, they are only present on the mother cell side of the invaginating sporulation septa. We provide evidence suggesting that the different distribution and number of FtsAZ filaments impact septal thickness, causing vegetative septa to be thicker than sporulation septa already during constriction. Finally, we show that a sporulation-specific protein, SpoIIE, regulates asymmetric divisome localization and septal thickness during sporulation.
External linksElife / PubMed:34018921 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-23963:
Tomogram of a dividing vegetative cell of Bacillus subtilis (Figure 3A of the manuscript Khanna et al., 2021)
Method: EM (tomography)

EMDB-23964:
Tomogram of a dividing vegetative cell of Bacillus subtilis (Figure 4A of the manuscript Khanna et al., 2021)
Method: EM (tomography)

EMDB-23965:
Tomogram of a dividing vegetative cell of Bacillus subtilis FtsZ-linker(Q-rich) strain (Figure 5A and 6A of the manuscript Khanna et al., 2021)
Method: EM (tomography)

EMDB-23966:
Tomogram of a dividing sporulating cell of Bacillus subtilis (Figure 7A of the manuscript Khanna et al., 2021)
Method: EM (tomography)

EMDB-23967:
Tomogram of a dividing sporulating cell of Bacillus subtilis (Figure 7 - figure supplement 4 of the manuscript Khanna et al., 2021)
Method: EM (tomography)

EMDB-23968:
Tomogram of a dividing sporulating cell of Bacillus subtilis SpoIIE null mutant (Figure 8A of the manuscript Khanna et al., 2001)
Method: EM (tomography)

Source
  • Bacillus subtilis PY79 (bacteria)

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