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TitleThe respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.
Journal, issue, pagesJ Gen Virol, Vol. 94, Issue Pt 8, Page 1734-1738, Year 2013
Publish dateMay 15, 2013
AuthorsSaskia E Bakker / Stéphane Duquerroy / Marie Galloux / Colin Loney / Edward Conner / Jean-François Eléouët / Félix A Rey / David Bhella /
PubMed AbstractRespiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical ...Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription.
External linksJ Gen Virol / PubMed:23677789 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Structure data

EMDB-2369, PDB-4bkk:
The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.
Method: EM (subtomogram averaging)

EMDB-2370:
The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.
Method: EM (subtomogram averaging)

Source
  • human respiratory syncytial virus a strain long
KeywordsVIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA COMPLEX

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