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TitleSubunit folds and maturation pathway of a dsRNA virus capsid.
Journal, issue, pagesStructure, Vol. 21, Issue 8, Page 1374-1383, Year 2013
Publish dateAug 6, 2013
AuthorsDaniel Nemecek / Evzen Boura / Weimin Wu / Naiqian Cheng / Pavel Plevka / Jian Qiao / Leonard Mindich / J Bernard Heymann / James H Hurley / Alasdair C Steven /
PubMed AbstractThe cystovirus ϕ6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits ...The cystovirus ϕ6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. ϕ6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an α-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.
External linksStructure / PubMed:23891288 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.5964 - 7.5 Å
Structure data

2364

4btg

EMDB-2364: CryoEM reconstruction of the bacteriophage phi6 procapsid to the near-atomic resolution
PDB-4btg: Coordinates of the bacteriophage phi6 capsid subunits (P1A and P1B) fitted into the cryoEM reconstruction of the procapsid at 4.4 A resolution
Method: EM (single particle) / Resolution: 4.4 Å

PDB-4btq:
Coordinates of the bacteriophage phi6 capsid subunits fitted into the cryoEM map EMD-1206
Method: ELECTRON MICROSCOPY / Resolution: 7.5 Å

PDB-4k7h:
Major capsid protein P1 of the Pseudomonas phage phi6
Method: X-RAY DIFFRACTION / Resolution: 3.5964 Å

Source
  • pseudomonas phage phi6 (bacteriophage)
KeywordsVIRUS / CYSTOVIRIDAE / PROCAPSID STRUCTURE / FLEXIBLE FITTING / VIRAL PROTEIN / major capsid protein

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