Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Heat-dependent opening of TRPV1 in the presence of capsaicin.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 28, Issue 7, Page 554-563, Year 2021
Publish date	Jul 8, 2021
Authors	Do Hoon Kwon / Feng Zhang / Yang Suo / Jonathan Bouvette / Mario J Borgnia / Seok-Yong Lee /
PubMed Abstract	Transient receptor potential vanilloid member 1 (TRPV1) is a Ca-permeable cation channel that serves as the primary heat and capsaicin sensor in humans. Using cryo-EM, we have determined the ...Transient receptor potential vanilloid member 1 (TRPV1) is a Ca-permeable cation channel that serves as the primary heat and capsaicin sensor in humans. Using cryo-EM, we have determined the structures of apo and capsaicin-bound full-length rat TRPV1 reconstituted into lipid nanodiscs over a range of temperatures. This has allowed us to visualize the noxious heat-induced opening of TRPV1 in the presence of capsaicin. Notably, noxious heat-dependent TRPV1 opening comprises stepwise conformational transitions. Global conformational changes across multiple subdomains of TRPV1 are followed by the rearrangement of the outer pore, leading to gate opening. Solvent-accessible surface area analyses and functional studies suggest that a subset of residues form an interaction network that is directly involved in heat sensing. Our study provides a glimpse of the molecular principles underlying noxious physical and chemical stimuli sensing by TRPV1, which can be extended to other thermal sensing ion channels.
External links	Nat Struct Mol Biol / PubMed:34239123 / PubMed Central
Methods	EM (single particle)
Resolution	2.63 - 3.72 Å
Structure data	23473 7lp9 EMDB-23473, PDB-7lp9: Cryo-EM structure of full-length TRPV1 at 4 degrees Celsius Method: EM (single particle) / Resolution: 2.63 Å 23474 7lpa EMDB-23474, PDB-7lpa: Cryo-EM structure of full-length TRPV1 with capsaicin at 4 degrees Celsius Method: EM (single particle) / Resolution: 3.37 Å 23475 7lpb EMDB-23475, PDB-7lpb: Cryo-EM structure of full-length TRPV1 with capsaicin at 25 degrees Celsius Method: EM (single particle) / Resolution: 3.54 Å 23476 7lpc EMDB-23476, PDB-7lpc: Cryo-EM structure of full-length TRPV1 at 48 degrees Celsius Method: EM (single particle) / Resolution: 3.06 Å EMDB-23477: Cryo-EM map of full-length TRPV1 with capsaicin at 48 degrees Celsius (10s), in an intermediate state Method: EM (single particle) / Resolution: 3.7 Å 23478 7lpd EMDB-23478, PDB-7lpd: Cryo-EM structure of full-length TRPV1 with capsaicin at 48 degrees Celsius, in an intermediate state, class 2 Method: EM (single particle) / Resolution: 3.55 Å 23479 7lpe EMDB-23479, PDB-7lpe: Cryo-EM structure of full-length TRPV1 with capsaicin at 48 degrees Celsius, in an open state, class 1 Method: EM (single particle) / Resolution: 3.72 Å
Chemicals	ChemComp-T7X: Phosphatidylinositol ChemComp-LBN: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / phospholipidYM ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / phospholipidYM ChemComp-NA: Unknown entry ChemComp-YFP: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol ChemComp-4DY: (6E)-N-(4-hydroxy-3-methoxybenzyl)-8-methylnon-6-enamide / neurotoxin*YM
Source	rattus norvegicus (Norway rat)
Keywords	MEMBRANE PROTEIN / Heat sensing ion channel