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TitleStructural Assembly of Qβ Virion and Its Diverse Forms of Virus-like Particles.
Journal, issue, pagesViruses, Vol. 14, Issue 2, Year 2022
Publish dateJan 24, 2022
AuthorsJeng-Yih Chang / Karl V Gorzelnik / Jirapat Thongchol / Junjie Zhang /
PubMed AbstractThe coat proteins (CPs) of single-stranded RNA bacteriophages (ssRNA phages) directly assemble around the genomic RNA (gRNA) to form a near-icosahedral capsid with a single maturation protein (Mat) ...The coat proteins (CPs) of single-stranded RNA bacteriophages (ssRNA phages) directly assemble around the genomic RNA (gRNA) to form a near-icosahedral capsid with a single maturation protein (Mat) that binds the gRNA and interacts with the retractile pilus during infection of the host. Understanding the assembly of ssRNA phages is essential for their use in biotechnology, such as RNA protection and delivery. Here, we present the complete gRNA model of the ssRNA phage Qβ, revealing that the 3' untranslated region binds to the Mat and the 4127 nucleotides fold domain-by-domain, and is connected through long-range RNA-RNA interactions, such as kissing loops. Thirty-three operator-like RNA stem-loops are located and primarily interact with the asymmetric A/B CP-dimers, suggesting a pathway for the assembly of the virions. Additionally, we have discovered various forms of the virus-like particles (VLPs), including the canonical = 3 icosahedral, larger = 4 icosahedral, prolate, oblate forms, and a small prolate form elongated along the 3-fold axis. These particles are all produced during a normal infection, as well as when overexpressing the CPs. When overexpressing the shorter RNA fragments encoding only the CPs, we observed an increased percentage of the smaller VLPs, which may be sufficient to encapsidate a shorter RNA.
External linksViruses / PubMed:35215818 / PubMed Central
MethodsEM (single particle)
Resolution4.6 - 8.9 Å
Structure data

23321

7lge

EMDB-23321: Phage Qbeta T=4 particle
PDB-7lge: Asymmetric unit for phage Qbeta T=4 particle
Method: EM (single particle) / Resolution: 5.6 Å

23322

7lgf

EMDB-23322: Phage Qbeta prolate particle
PDB-7lgf: Asymmetric unit for phage Qbeta prolate particle
Method: EM (single particle) / Resolution: 6.1 Å

23323

7lgg

EMDB-23323: Phage Qbeta oblate particle
PDB-7lgg: Asymmetric unit for phage Qbeta oblate particle
Method: EM (single particle) / Resolution: 6.2 Å

23324

7lgh

EMDB-23324: Phage Qbeta small prolate particle
PDB-7lgh: Asymmetric unit for phage Qbeta small prolate particle
Method: EM (single particle) / Resolution: 8.9 Å

23336

7lhd

EMDB-23336: Phage Qbeta virion
PDB-7lhd: The complete model of phage Qbeta virion
Method: EM (single particle) / Resolution: 4.6 Å

Source
  • escherichia phage qbeta (virus)
  • Bacteriophage Q-beta (virus)
  • escherichia virus qbeta
KeywordsVIRUS LIKE PARTICLE / Bacteriophage / Virus / Qbeta / prolate / oblate / Small prolate / VIRUS/RNA / Virion / VIRUS-RNA complex

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