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TitleTunable Heteroassembly of a Plant Pseudoenzyme-Enzyme Complex.
Journal, issue, pagesACS Chem Biol, Vol. 16, Issue 11, Page 2315-2325, Year 2021
Publish dateNov 19, 2021
AuthorsIrina V Novikova / Mowei Zhou / Chen Du / Marcelina Parra / Doo Nam Kim / Zachary L VanAernum / Jared B Shaw / Hanjo Hellmann / Vicki H Wysocki / James E Evans /
PubMed AbstractPseudoenzymes have emerged as key regulatory elements in all kingdoms of life despite being catalytically nonactive. Yet many factors defining why one protein is active while its homologue is ...Pseudoenzymes have emerged as key regulatory elements in all kingdoms of life despite being catalytically nonactive. Yet many factors defining why one protein is active while its homologue is inactive remain uncertain. For pseudoenzyme-enzyme pairs, the similarity of both subunits can often hinder conventional characterization approaches. In plants, a pseudoenzyme, PDX1.2, positively regulates vitamin B production by association with its active catalytic homologues such as PDX1.3 through an unknown assembly mechanism. Here we used an integrative experimental approach to learn that such pseudoenzyme-enzyme pair associations result in heterocomplexes of variable stoichiometry, which are unexpectedly tunable. We also present the atomic structure of the PDX1.2 pseudoenzyme as well as the population averaged PDX1.2-PDX1.3 pseudoenzyme-enzyme pair. Finally, we dissected hetero-dodecamers of each stoichiometry to understand the arrangement of monomers in the heterocomplexes and identified symmetry-imposed preferences in PDX1.2-PDX1.3 interactions. Our results provide a new model of pseudoenzyme-enzyme interactions and their native heterogeneity.
External linksACS Chem Biol / PubMed:34520180 / PubMed Central
MethodsEM (single particle)
Resolution3.16 Å
Structure data

23263

7lb5

EMDB-23263: Cryo-EM map of pyridoxal 5'-phosphate synthase-like subunit PDX1.2 (Arabidopsis thaliana)
PDB-7lb5: Pyridoxal 5'-phosphate synthase-like subunit PDX1.2 (Arabidopsis thaliana)
Method: EM (single particle) / Resolution: 3.16 Å

23264

7lb6

EMDB-23264: Cryo-EM map of PDX1.2/PDX1.3 co-expression complex (Arabidopsis thaliana)
PDB-7lb6: PDX1.2/PDX1.3 co-expression complex
Method: EM (single particle) / Resolution: 3.16 Å

Source
  • arabidopsis thaliana (thale cress)
KeywordsPLANT PROTEIN / pseudoenzyme / dodecamer / vitamin B6

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