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TitleMicrochip-Based Structure Determination of Disease-Relevant p53.
Journal, issue, pagesAnal Chem, Vol. 92, Issue 23, Page 15558-15564, Year 2020
Publish dateDec 1, 2020
AuthorsMaria J Solares / G M Jonaid / William Y Luqiu / Yanping Liang / Madison C Evans / William J Dearnaley / Zhi Sheng / Deborah F Kelly /
PubMed AbstractThe tumor suppressor protein TP53 (p53) plays a multifaceted role in all cells of the human body. Mutations in the gene are often involved in cancer induction and disease progression. Despite its ...The tumor suppressor protein TP53 (p53) plays a multifaceted role in all cells of the human body. Mutations in the gene are often involved in cancer induction and disease progression. Despite its important role in health and development, structural information for p53 remains incomplete. Here, we present a microchip-based technology to facilitate structural studies of p53 assemblies derived from human cancer cells. These devices do not introduce foreign sequences to the gene and maintain naturally occurring post-translational modifications. Using cryo-electron microscopy, structures for the p53 monomer (∼50 kDa) and tetramer (∼200 kDa) were resolved to ∼4.8 and ∼7 Å, respectively. These structures revealed new insights for flexible regions of p53 along with biologically relevant ubiquitination sites. Collectively, the convergence of nanotechnology tools and structural imaging builds a strong framework to understand the oncogenic impact of p53 in human tissues.
External linksAnal Chem / PubMed:33124814 / PubMed Central
MethodsEM (single particle)
Resolution7.0 Å
Structure data

EMDB-22827:
P53 tetramer from Glioblastoma
Method: EM (single particle) / Resolution: 7.0 Å

Source
  • Homo sapiens (human)

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