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Structure paper

TitleStructures of radial spokes and associated complexes important for ciliary motility.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 1, Page 29-37, Year 2021
Publish dateDec 14, 2020
AuthorsMiao Gui / Meisheng Ma / Erica Sze-Tu / Xiangli Wang / Fujiet Koh / Ellen D Zhong / Bonnie Berger / Joseph H Davis / Susan K Dutcher / Rui Zhang / Alan Brown /
PubMed AbstractIn motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two ...In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform.
External linksNat Struct Mol Biol / PubMed:33318703 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 6.3 Å
Structure data

22475

7jtk

EMDB-22475: Composite cryo-EM density map of the radial spoke 1 isolated from Chlamydomonas reinhardtii
PDB-7jtk: Radial spoke 1 isolated from Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 3.2 Å

22480

7jts

EMDB-22480: Cryo-EM density map of stalk of radial spoke 1 attached with doublet microtubule from Chlamydomonas reinhardtii
PDB-7jts: Stalk of radial spoke 1 attached with doublet microtubule from Chlamydomonas reinhardtii
Method: EM (single particle) / Resolution: 6.1 Å

22481

7ju4

EMDB-22481: Composite cryo-EM density map of radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule
PDB-7ju4: Radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-22482:
Cryo-EM density map of on-doublet radial spoke 1 head
Method: EM (single particle) / Resolution: 5.3 Å

EMDB-22483:
Cryo-EM density map of on-doublet radial spoke 2 head
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-22486:
Cryo-EM density map of RSP1 dimer from two radial spokes
Method: EM (single particle) / Resolution: 6.3 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • chlamydomonas reinhardtii (plant)
KeywordsSTRUCTURAL PROTEIN / cilia / native / complex / mechanoregulation / microtubule

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