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Structure paper

TitleAtomic structures of respiratory complex III, complex IV, and supercomplex III-IV from vascular plants.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateJan 19, 2021
AuthorsMaria Maldonado / Fei Guo / James A Letts /
PubMed AbstractMitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes ...Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII, CIV, and SC III+IV from determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII revealed long-range, coordinated movements across the complex, as well as the motion of CIII's iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes and generate new mechanistic hypotheses.
External linksElife / PubMed:33463523 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.8 Å
Structure data

22445

7jrg

EMDB-22445, PDB-7jrg:
Plant Mitochondrial complex III2 from Vigna radiata
Method: EM (single particle) / Resolution: 3.2 Å

22447

7jro

EMDB-22447, PDB-7jro:
Plant Mitochondrial complex IV from Vigna radiata
Method: EM (single particle) / Resolution: 3.8 Å

22448

7jrp

EMDB-22448: Plant Mitochondrial complex SC III2+IV from Vigna radiata composite map
PDB-7jrp: Plant Mitochondrial complex SC III2+IV from Vigna radiata
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-22449:
Plant mitochondrial supercomplex III2+IV from Vigna radiata
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-22450:
Plant mitochondrial complex III2 from Vigna radiata supercomplex III2+IV, focused refinement.
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

ChemComp-HEC:
HEME C

ChemComp-HEA:
HEME-A

ChemComp-CU:
COPPER (II) ION

ChemComp-MG:
Unknown entry

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-LYS:
LYSINE

Source
  • vigna radiata (mung bean)
  • Mung bean (mung bean)
  • vigna radiata var. radiata (mung bean)
KeywordsELECTRON TRANSPORT / mitochondria / respiration / bioenergetics / plants / OXIDOREDUCTASE

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